Genetic variation among accessions of the genus Manihot by ISSR markers

Authors

  • Kaliny Veiga Pessoa da silva UFRPE
  • Alfredo Augusto da Cunha Alves Embrapa-Labex
  • Maria Isabel Gomes Martins UFRPE
  • Cláusio Antônio Ferreira de Melo State University of Santa Cruz image/svg+xml
  • Reginaldo de Carvalho UFRPE

DOI:

https://doi.org/10.1590/S1678-3921.pab2011.v46.10850

Keywords:

germplasm, genetic diversity, cassava, polymorphism.

Abstract

The objective of this work was to evaluate the genetic diversity within and among accessions of Manihot by using ISSR markers. Five species and two varieties of Manihot, besides two species of the genus Croton, used as the out-group, were analyzed by using 20 oligonucleotide (Olii) ISSR UBC primers. To analyze the similarity index between species and accessions, the Jaccard and simple matching coefficients were used. The 20 Olii tested were highly polymorphic in all species analyzed, and 89.7% of the loci were polymorphic. A higher genetic variability is observed among different species of Manihot, such as M. dichotoma var. undulata and M. caerulescens, than among individuals of the same species, such as M. dichotoma and M. dichotoma var. undulata.

Author Biographies

  • Kaliny Veiga Pessoa da silva, UFRPE
    http://lattes.cnpq.br/0579417795319098
  • Maria Isabel Gomes Martins, UFRPE
    http://lattes.cnpq.br/8446164424520637
  • Cláusio Antônio Ferreira de Melo, State University of Santa Cruz
    http://lattes.cnpq.br/4143311508589631
  • Reginaldo de Carvalho, UFRPE
    http://lattes.cnpq.br/5208573547132664

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Published

2011-12-14

Issue

v.46, n.9, set. 2011

Section

GENETICS

How to Cite

Pessoa da silva, K. V., Alves, A. A. da C., Martins, M. I. G., de Melo, C. A. F., & de Carvalho, R. (2011). Genetic variation among accessions of the genus Manihot by ISSR markers. Pesquisa Agropecuaria Brasileira, 46(9), 1082-1088. https://doi.org/10.1590/S1678-3921.pab2011.v46.10850