Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera)

Authors

  • Diego Herman Sauka Instituto Nacional de Tecnología Agropecuaria, Instituto de Microbiología y Zoología Agrícola, Insumos Bacterianos, De los Reseros y Las Cabañas
  • Rodrigo Horacio Monella Instituto Nacional de Tecnología Agropecuaria, Instituto de Microbiología y Zoología Agrícola, Insumos Bacterianos, De los Reseros y Las Cabañas
  • Graciela Beatriz Benintende Instituto Nacional de Tecnología Agropecuaria, Instituto de Microbiología y Zoología Agrícola, Insumos Bacterianos, De los Reseros y Las Cabañas

DOI:

https://doi.org/10.1590/S1678-3921.pab2010.v45.7823

Keywords:

biological control, Coccinellidae, Cucurbitaceae, ladybird beetle, spore-crystal toxicity

Abstract

The objective of this work was to establish and test the induced-feeding bioassay in order to detect Bacillus thuringiensis insecticidal activity against (Coleoptera: Coccinellidae). Larvae were induced to swallow high concentrations of spore-crystal suspensions of s Argentine B. thuringiensis strains. The great majority of strains showed no toxicity, and observed mortality was lower than 30%. Induced-feeding bioassay is feasible, and should be used for prospecting strains that produce right combinations of Cry proteins needed to an efficient pest control.

Author Biography

  • Diego Herman Sauka, Instituto Nacional de Tecnología Agropecuaria, Instituto de Microbiología y Zoología Agrícola, Insumos Bacterianos, De los Reseros y Las Cabañas

    Investigador

    Instituto de Microbiología y Zoología Agrícola, INTA

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Published

2011-01-18

Issue

v.45, n.4, abr. 2010

Section

SCIENTIFIC NOTES

How to Cite

Sauka, D. H., Monella, R. H., & Benintende, G. B. (2011). Induced-feeding bioassays for detection of Bacillus thuringiensis insecticidal activity against Epilachna paenulata (Coleoptera). Pesquisa Agropecuaria Brasileira, 45(4), 430-432. https://doi.org/10.1590/S1678-3921.pab2010.v45.7823