Bovine herpesvirus type 1 in cumulus‑oocyte complexes collected from naturally infected cows

Authors

  • Andre Penido Oliveira Empresa de Pesquisa Agropecuária de Minas Gerais, Rua Afonso Rato, no 1.301, Mercês, CEP 38001‑970 Uberaba, MG.
  • Marcos Bryan Heinemann Universidade Federal de Minas Gerais, Escola de Veterinária, Campus Pampulha, Avenida Antônio Carlos, no 6.627, CEP 31270‑901 Belo Horizonte, MG.
  • Adriana Cortez Universidade Santo Amaro, Campus I, Rua Professor Enéas de Siqueira Neto, no 340, Jardim das Imbuias, CEP 04829‑300 São Paulo, SP.
  • Paula Maria Pires Nascimento Universidade Federal de Minas Gerais, Escola de Veterinária, Campus Pampulha, Avenida Antônio Carlos, no 6.627, CEP 31270‑901 Belo Horizonte, MG.
  • Romulo Cerqueira Leite Ribeiro Universidade Federal de Minas Gerais, Escola de Veterinária, Campus Pampulha, Avenida Antônio Carlos, no 6.627, CEP 31270‑901 Belo Horizonte, MG.
  • Joao Henrique Moreira Viana Embrapa Gado de Leite, Rua Eugênio do Nascimento, no 610, Dom Bosco, CEP 36038‑330 Juiz de Fora, MG.

DOI:

https://doi.org/10.1590/S1678-3921.pab2016.v51.20775

Keywords:

Bos indicus, follicular fluid, infectious bovine rhinotracheitis virus, transmission risk, viral DNA

Abstract

The objective of this work was to investigate the presence of bovine herpesvirus type 1 (BoHV‑1) in follicular fluid and in cumulus‑oocyte complexes (COC) recovered from naturally infected cows but with no clinical signs of the disease. Cows that were seropositive (n=38) or seronegative (n=8, control) to infectious bovine rhinotracheitis were selected after a serum neutralization test in microplates. The presence of the virus was investigated by PCR in COC and in follicular fluid. Viral DNA was not found in any of the samples. The obtained results suggest that serologically positive cows with no clinical signs of the disease offer negligible risk of transmitting BoHV‑1 by COC or follicular fluid.

Author Biographies

Andre Penido Oliveira, Empresa de Pesquisa Agropecuária de Minas Gerais, Rua Afonso Rato, no 1.301, Mercês, CEP 38001‑970 Uberaba, MG.


Adriana Cortez, Universidade Santo Amaro, Campus I, Rua Professor Enéas de Siqueira Neto, no 340, Jardim das Imbuias, CEP 04829‑300 São Paulo, SP.


Paula Maria Pires Nascimento, Universidade Federal de Minas Gerais, Escola de Veterinária, Campus Pampulha, Avenida Antônio Carlos, no 6.627, CEP 31270‑901 Belo Horizonte, MG.


Joao Henrique Moreira Viana, Embrapa Gado de Leite, Rua Eugênio do Nascimento, no 610, Dom Bosco, CEP 36038‑330 Juiz de Fora, MG.


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Published

2016-08-03

How to Cite

Oliveira, A. P., Heinemann, M. B., Cortez, A., Nascimento, P. M. P., Ribeiro, R. C. L., & Viana, J. H. M. (2016). Bovine herpesvirus type 1 in cumulus‑oocyte complexes collected from naturally infected cows. Pesquisa Agropecuaria Brasileira, 51(5), 676–679. https://doi.org/10.1590/S1678-3921.pab2016.v51.20775

Issue

v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária

Section

SCIENTIFIC NOTES