Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States

Authors

  • Stephanie Regina Alves Botelho Universidade Católica de Brasília, QS 07, Lote 01, EPCT, CEP 71966‑700 Águas Claras, DF.
  • Macária Ferreira Duarte Faculdade Anhanguera, QS 1, Rua 212, Lotes 11, 13 e 15, CEP 71950‑550 Águas Claras, DF.
  • Andreza Viveiros Barbosa Faculdade Anhanguera, QS 1, Rua 212, Lotes 11, 13 e 15, CEP 71950‑550 Águas Claras, DF.
  • Douglas Lau Embrapa Trigo, Rodovia BR‑285, Km 294, CEP 99050‑970 Passo Fundo, RS.
  • Márcio Martinello Sanches Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Avenida W5 Norte (Final), Caixa Postal 02372, CEP 70770‑917 Brasília, DF.
  • Fernanda Rausch Fernandes Embrapa Quarentena Vegetal, Parque Estação Biológica, s/no, CEP 70770‑901 Brasília, DF.

DOI:

https://doi.org/10.1590/S1678-3921.pab2016.v51.20981

Keywords:

Zea mays, exotic pest, High plains virus, plant quarantine, phytosanitary analysis

Abstract

The objective of this work was to evaluate the phytosanitary aspect of two accessions of maize (Zea mays) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real‑time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The obtained results show WMoV interception in Brazil.

Author Biography

Fernanda Rausch Fernandes, Embrapa Quarentena Vegetal, Parque Estação Biológica, s/no, CEP 70770‑901 Brasília, DF.

Pesquisadora A em Virologia Vegetal da Embrapa Quarentena Vegetal

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Published

2016-08-03

How to Cite

Botelho, S. R. A., Duarte, M. F., Barbosa, A. V., Lau, D., Sanches, M. M., & Fernandes, F. R. (2016). Interception of <i>Wheat mosaic virus</i> (WMoV) in Brazil in maize seeds from the United States. Pesquisa Agropecuaria Brasileira, 51(5), 688–691. https://doi.org/10.1590/S1678-3921.pab2016.v51.20981

Issue

v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária

Section

SCIENTIFIC NOTES