Viability of dourado embryos cooled in different cryoprotectant solutions

Authors

  • Darci Carlos Fornari Universidade Federal do Rio Grande do Sul
  • Danilo Pedro Streit Junior Universidade Federal do Rio Grande do Sul
  • Juliana Minardi Galo Universidade Estadual de Maringá
  • Luís Jayme Guerreiro Universidade Federal do Rio Grande do Sul
  • Jayme Aparecido Povh Universidade Federal do Mato Grosso do Sul
  • Nelson Mauricio Lopera Barrero Universidade Estadual de Londrina
  • Diego de Oliveira Universidade Federal do Rio Grande do Sul
  • Ricardo Pereira Ribeiro Universidade Estadual de Maringá

DOI:

https://doi.org/10.1590/S1678-3921.pab2013.v48.11186

Keywords:

Salminus brasiliensis, cryopreservation, fish embryo, embryo preservation, larvae eclosion

Abstract

The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested. For each solution, 300 embryos were selected at the closing of the blastopore stage, and 300 more embryos were used as a negative control. After cooling (‑8°C for 6 hours), the embryos were rehydrated directly in the incubator until hatching. The best result is obtained with the cryoprotectant solution containing 9% methanol associated with 17% sucrose, resulting in a larvae hatching rate of 67.06%.

Author Biographies

Danilo Pedro Streit Junior, Universidade Federal do Rio Grande do Sul

Ricardo Pereira Ribeiro, Universidade Estadual de Maringá

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Published

2013-10-31

How to Cite

Fornari, D. C., Streit Junior, D. P., Galo, J. M., Guerreiro, L. J., Povh, J. A., Barrero, N. M. L., … Ribeiro, R. P. (2013). Viability of dourado embryos cooled in different cryoprotectant solutions. Pesquisa Agropecuaria Brasileira, 48(8), 1175–1178. https://doi.org/10.1590/S1678-3921.pab2013.v48.11186

Issue

v.48, n.8, ago. 2013: Thematic Issue Aquaculture

Section

SCIENTIFIC NOTES