Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie
DOI:
https://doi.org/10.1590/S1678-3921.pab2016.v51.20804Keywords:
genotyping, PRNP gene, single nucleotide polymorphisms, transmissible spongiform encephalopathiesAbstract
The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.Downloads
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Published
2016-08-03
How to Cite
Gonçalves, A. N. D., Soares, C. O., Sanches, S. C., Reis, F. A., & Rosinha, G. M. S. (2016). Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. Pesquisa Agropecuaria Brasileira, 51(5), 684–687. https://doi.org/10.1590/S1678-3921.pab2016.v51.20804