Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie

Authors

  • Aline Najara Domingos Gonçalves Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina Veterinária e Zootecnia, Avenida Senador Felinto Müller, no 2443, CEP 79070‑900 Campo Grande, MS.
  • Cleber Oliveira Soares Embrapa Gado de Corte, Avenida Rádio Maia, no 830, Zona Rural, CEP 79106‑550 Campo Grande, MS.
  • Simone Camargo Sanches Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina Veterinária e Zootecnia, Avenida Senador Felinto Müller, no 2443, CEP 79070‑900 Campo Grande, MS.
  • Fernando Alvarenga Reis Embrapa Caprinos e Ovinos, Estrada Sobral/Groaíras, Km 4, Caixa Postal 145, CEP 62010‑970 Sobral, CE.
  • Grácia Maria Soares Rosinha Embrapa Gado de Corte, Avenida Rádio Maia, no 830, Zona Rural, CEP 79106‑550 Campo Grande, MS.

DOI:

https://doi.org/10.1590/S1678-3921.pab2016.v51.20804

Keywords:

genotyping, PRNP gene, single nucleotide polymorphisms, transmissible spongiform encephalopathies

Abstract

The objective of this work was to determine the genotypic profile specific to scrapie in codons 136, 154, and 171 of the PRNP gene of the Pantanal creole sheep. Genomic DNA was extracted from blood samples collected from 66 sheep, and the regions of interest on the DNA strand were amplified by PCR. Five haplotypes were identified: ARR, alanine, arginine, arginine; ARQ, alanine, arginine, glutamine; AHQ, alanine, histidine, glutamine; ARH, alanine, arginine, histidine; and VRQ, valine, arginine, glutamine. The most common genotypes were ARQ/ARQ (27%) and ARR/ARQ (24%). The genotypic profile of the Pantanal creole sheep shows low to moderate susceptibility.

Author Biographies

Aline Najara Domingos Gonçalves, Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina Veterinária e Zootecnia, Avenida Senador Felinto Müller, no 2443, CEP 79070‑900 Campo Grande, MS.


Cleber Oliveira Soares, Embrapa Gado de Corte, Avenida Rádio Maia, no 830, Zona Rural, CEP 79106‑550 Campo Grande, MS.


Simone Camargo Sanches, Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina Veterinária e Zootecnia, Avenida Senador Felinto Müller, no 2443, CEP 79070‑900 Campo Grande, MS.


Fernando Alvarenga Reis, Embrapa Caprinos e Ovinos, Estrada Sobral/Groaíras, Km 4, Caixa Postal 145, CEP 62010‑970 Sobral, CE.


Grácia Maria Soares Rosinha, Embrapa Gado de Corte, Avenida Rádio Maia, no 830, Zona Rural, CEP 79106‑550 Campo Grande, MS.


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Published

2016-08-03

How to Cite

Gonçalves, A. N. D., Soares, C. O., Sanches, S. C., Reis, F. A., & Rosinha, G. M. S. (2016). Genotypic profile of Pantanal creole sheep regarding susceptibility or resistance to scrapie. Pesquisa Agropecuaria Brasileira, 51(5), 684–687. https://doi.org/10.1590/S1678-3921.pab2016.v51.20804