Effect of GA3 and active charcoal on the in vitro rooting of 'Natal' sweet orange

Abstract

Embryos of 'Natal' sweet orange (Citrus sinensis Osb.) were excised from fruits ten weeks after poilination. They were classified by size ( < 3 mm, 3-6 mm and > 6 mm) and cultivated in vitro. The "MS" media were used without growth regulators, including combinations of gibberellic acid (0.0, 0,01, 0.1 and 1.0 mg/l) and active charcoal (0.0, 0.3 and 1.0 gil), with pH 53 and agar 7 g/l. The highest frequency of rooting was observed in gibberellic acid 0.01 and 1.0 mgil with active charcoal 1.0 g/l. The best root development was observed in gibberellic acid 1.0 mg/l and active charcoal 1.0 g/l.