In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation

Authors

  • Hugo Teixeira Gomes Universidade de Brasília, Departamento de Botânica, Avenida L3 Norte s/no, CEP 70910-900 Brasília, DF.
  • Patrícia Monah Cunha Bartos Universidade de Brasília, Departamento de Botânica, Avenida L3 Norte s/no, CEP 70910-900 Brasília, DF.
  • Maíra Teixeira de Andrade Universidade de Brasília, Departamento de Botânica, Avenida L3 Norte s/no, CEP 70910-900 Brasília, DF.
  • Raphael Ferreira Almeida Universidade de Brasília, Departamento de Botânica, Avenida L3 Norte s/no, CEP 70910-900 Brasília, DF.
  • Luciana Florencio de Lacerda Universidade de Brasília, Departamento de Botânica, Avenida L3 Norte s/no, CEP 70910-900 Brasília, DF.
  • Jonny Everson Scherwinski-Pereira Embrapa Recursos Genéticos e Biotecnologia, Avenida W5 Norte (Final), CEP 70770-917 Brasília, DF.

DOI:

https://doi.org/10.1590/S1678-3921.pab2017.v52.24722

Keywords:

Rubus, ex situ conservation, plant genetic resources, plant propagation, slow-growth

Abstract

The objective of this work was to evaluate the micropropagation of blackberry (Rubus spp.) cultivars, after in vitro conservation under minimal growth conditions. Nodal segments of the 'Guarani', 'Caingangue', 'Ébano', and 'Xavante' genotypes were conserved under minimal growth conditions at 20ºC, for 15 months. Microshoots were regenerated and multiplied by up to five successive subcultures, when they were rooted and acclimatized. After 30 days of acclimatization in a greenhouse, rooted plantlets showed no significant losses. Blackberry cultivars can be conserved in vitro for 15 months, without subcultures and, after this time, they can be micropropagated on a large-scale, maintaining the regenerative potential and multiplication.

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Published

2017-12-21

How to Cite

Gomes, H. T., Bartos, P. M. C., Andrade, M. T. de, Almeida, R. F., Lacerda, L. F. de, & Scherwinski-Pereira, J. E. (2017). In vitro conservation of blackberry genotypes under minimal growth conditions and subsequent large-scale micropropagation. Pesquisa Agropecuaria Brasileira, 52(12), 1286–1290. https://doi.org/10.1590/S1678-3921.pab2017.v52.24722

Issue

v.52, n.12, dez. 2017

Section

SCIENTIFIC NOTES