Bovine sperm cell motility after incubation in follicular fluid

Authors

  • Sergio Farias Vargas Júnior Universidade Federal de Pelotas, Faculdade de Veterinária, Campus Universitário, s/no, Prédio 1, Caixa Postal 354, CEP 96010-900 Capão do Leão, RS.
  • Vitória Gasperin Guazzelli Costa Universidade Federal de Pelotas, Faculdade de Veterinária, Campus Universitário, s/no, Prédio 1, Caixa Postal 354, CEP 96010-900 Capão do Leão, RS.
  • Fernando Caetano de Oliveira Universidade Federal de Pelotas, Faculdade de Veterinária, Campus Universitário, s/no, Prédio 1, Caixa Postal 354, CEP 96010-900 Capão do Leão, RS.
  • Karina Lemos Goularte Universidade Federal de Pelotas, Faculdade de Veterinária, Campus Universitário, s/no, Prédio 1, Caixa Postal 354, CEP 96010-900 Capão do Leão, RS.
  • Cristina Sangoi Haas Universidade Federal de Pelotas, Faculdade de Veterinária, Campus Universitário, s/no, Prédio 1, Caixa Postal 354, CEP 96010-900 Capão do Leão, RS.
  • Bernardo Garziera Gasperin Universidade Federal de Pelotas, Faculdade de Veterinária, Campus Universitário, s/no, Prédio 1, Caixa Postal 354, CEP 96010-900 Capão do Leão, RS.
  • Thomaz Lucia Júnior Universidade Federal de Pelotas, Faculdade de Veterinária, Campus Universitário, s/no, Prédio 1, Caixa Postal 354, CEP 96010-900 Capão do Leão, RS.

DOI:

https://doi.org/10.1590/S1678-3921.pab2018.v53.25943

Keywords:

infertility, insemination, intrafollicular artificial insemination, spermatozoa

Abstract

The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination (IFAI) in vivo or after incubation in follicular fluid in vitro. In the in vivo experiment, IFAI was performed, followed by the recovery of follicular content 1 to 4 hours later, in order to assess sperm motility. In the in vitro experiment, spermatozoa from a pool of commercial frozen-thawed semen were evaluated for their kinetics after incubation for 1 or 3 hours, either pure (pool, control group) or in follicular fluid (FF). A low motility of sperm cells was observed in the FF samples, both in vitro and in vivo. In vitro, the main parameters negatively affected in the sperm cells incubated in FF, compared with the control, were: total motility (TM), progressive motility (PM), curvilinear distance, and straightness, after 1 hour of incubation; and TM, PM, average path velocity, and curvilinear velocity after 3 hours of incubation. The ovarian follicle and follicular fluid do not provide a suitable environment to maintain bovine sperm cell motility.

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Published

2018-10-09

How to Cite

Vargas Júnior, S. F., Costa, V. G. G., Oliveira, F. C. de, Goularte, K. L., Haas, C. S., Gasperin, B. G., & Lucia Júnior, T. (2018). Bovine sperm cell motility after incubation in follicular fluid. Pesquisa Agropecuaria Brasileira, 53(7), 866–869. https://doi.org/10.1590/S1678-3921.pab2018.v53.25943

Issue

v.53, n.7, jul. 2018

Section

SCIENTIFIC NOTES