Diversity of <i>Pratylenchus zeae</i> populations in sugarcane mills in the Brazilian Northeast region
Keywords:
Saccharum, PCR, phylogeny, root-lesion nematode, variabilityAbstract
The objective of this work was to identity and characterize
morphometric, molecular, and phylogenetic features of Pratylenchus spp. populations collected from sugarcane mills in the Brazilian Northeast region. Thirty populations of Pratylenchus were analyzed morphologically, morphometrically, and molecularly using the D2-D3 28S rDNA region. Samples were collected from sugarcane mills in the states of Paraíba, Pernambuco, and Rio Grande do Norte. Morphological analysis of Pratylenchus spp. collected from sugarcane mills suggests that the species present in the Brazilian Northeast region is P. zeae. Morphometric data show overlap with previous described Pratylenchus species but support the identification of P. zeae as the species present in the analyzed samples. Molecular analysis confirms that all Pratylenchus populations collected from sugarcane mills in the Brazilian Northeast region belong to the species P. zeae. Soil clay content and chemical characteristics affect the morphometry of P. zeae populations.