Genetic characterization and population structure of Canela-Preta creole chicken

Autores

  • Débora Araújo de Carvalho Universidade Federal dos Vales do Jequitinhonha e Mucuri, Departamento de Zootecnia, Campus JK, Rodovia MGT 367, Km 583, no 5.000, Alto da Jacuba, CEP 39100-000 Diamantina, MG, Brasil.
  • Cristina Moreira Bonafe Universidade Federal dos Vales do Jequitinhonha e Mucuri, Departamento de Zootecnia, Campus JK, Rodovia MGT 367, Km 583, no 5.000, Alto da Jacuba, CEP 39100-000 Diamantina, MG, Brasil.
  • Maria Del Pilar Rodriguez-Rodriguez Universidade Federal dos Vales do Jequitinhonha e Mucuri, Departamento de Zootecnia, Campus JK, Rodovia MGT 367, Km 583, no 5.000, Alto da Jacuba, CEP 39100-000 Diamantina, MG, Brasil.
  • Marcos Jacob de Oliveira Almeida Embrapa Meio-Norte, Transferência de Tecnologia, Avenida Duque de Caxias, no 5.650, Buenos Aires, CEP 64006-245 Teresina, PI, Brasil.
  • José Lindenberg Rocha Sarmento Universidade Federal do Piauí, Departamento de Zootecnia e Ciências Biológicas, Campus Universitário Ministro Petrônio Portella, Ininga, s/no, CEP 64049-550 Teresina, PI, Brasil.
  • Fabio Barros Britto Universidade Federal do Piauí, Departamento de Zootecnia e Ciências Biológicas, Campus Universitário Ministro Petrônio Portella, Ininga, s/no, CEP 64049-550 Teresina, PI, Brasil.
  • Martinho de Almeida e Silva Universidade Federal dos Vales do Jequitinhonha e Mucuri, Departamento de Zootecnia, Campus JK, Rodovia MGT 367, Km 583, no 5.000, Alto da Jacuba, CEP 39100-000 Diamantina, MG, Brasil.

DOI:

https://doi.org/10.1590/S1678-3921.pab2016.v51.23879

Palavras-chave:

Gallus gallus, microsatellites, polymorphism, genetic variability

Resumo

The objective of this work was to genetically characterize and evaluate the population structure of Canela-Preta creole chicken of three breeding stocks belonging to the municipalities of Teresina, Oeiras and Queimada Nova, in the state of Piauí, Brazil. Twelve microsatellite markers and DNA samples from
118 chickens were used. After DNA extraction, the microsatellite markers were amplified by polymerase chain reaction (PCR). Statistical analyses of the observed and expected heterozygosity estimates, analysis of molecular variance, principal components analysis, Wright’s F-statistics, and analysis of population structure based on Bayesian analysis were performed. The analyses of genetic differentiation (using Amova) suggested low differentiation between the evaluated nucleuses, indicating this group as genetically unique. The results
of the F-statistics indicated endogamy trend of the breeding stocks studied. Scatter plot and Bayesian analysis, used to show the structure of Canela-Preta birds, suggested the existence of four genetic groups and revealed that there is gene flow between the breeding stocks analysed. The evaluated microsatellite molecular markers showed to be polymorphic, which shows high variation in the samples and reveals their effectiveness in the study of characterization. The results indicate that Canela-Preta chickens are genetically structured.

Biografia do Autor

Maria Del Pilar Rodriguez-Rodriguez, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Departamento de Zootecnia, Campus JK, Rodovia MGT 367, Km 583, no 5.000, Alto da Jacuba, CEP 39100-000 Diamantina, MG, Brasil.

 

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Publicado

2016-12-23

Como Citar

Carvalho, D. A. de, Bonafe, C. M., Rodriguez-Rodriguez, M. D. P., Almeida, M. J. de O., Sarmento, J. L. R., Britto, F. B., & Silva, M. de A. e. (2016). Genetic characterization and population structure of Canela-Preta creole chicken. Pesquisa Agropecuária Brasileira, 51(11), 1899–1906. https://doi.org/10.1590/S1678-3921.pab2016.v51.23879

Edição

v.51, n.11, nov. 2016

Seção

ZOOTECNIA