Mannitol for coconut ex situ conservation by minimum growth

Authors

  • Ana da Silva Lédo Embrapa Tabuleiros Costeiros
  • Catrine Regina Feitosa Moura Federal University of Sergipe image/svg+xml
  • Caroline de Araújo Machado
  • Semíramis Rabelo Ramalho Ramos Embrapa Tabuleiros Costeiros
  • Ana Veruska Cruz da Silva Embrapa Tabuleiros Costeiros
  • Carlos Alberto da Silva Lédo Embrapa Cassava & Fruits image/svg+xml

DOI:

https://doi.org/10.1590/S1678-3921.pab2014.v49.17435

Keywords:

Cocos nucifera, germplasm, osmotic regulator, tissue culture

Abstract

The objective of this work was to determine the mannitol concentration for in vitro germination and minimum growth of coconut (Cocos nucifera). The following accessions were used: Brazilian Red Dwarf Gramame (BRDG), Malaysian Yellow Dwarf (MYD), Brazilian Tall (BRA), Polynesian Tall (PYT), and Rennell Island Tall (RIT). Embryos were inoculated in an Y3 culture medium at different mannitol concentrations (0, 0.1, 0.2, 0.3, and 0.4 mol L‑1). Inhibition of shoot development was verified at 0.1, 0.2, and 0.3 mol L‑1, for MYD and BRDG, and at 0.1 and 0.2 mol L‑1, for PYT, BRA, and RIT. Mannitol use is a promising strategy for minimum growth conservation.

Author Biographies

  • Ana da Silva Lédo, Embrapa Tabuleiros Costeiros
    http://lattes.cnpq.br/8253115718345940 Aracaju, SE
  • Caroline de Araújo Machado
    Universidade Federal de Sergipe

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Published

2014-05-08

Issue

v.49, n.2, fev. 2014

Section

SCIENTIFIC NOTES

How to Cite

Lédo, A. da S., Moura, C. R. F., Machado, C. de A., Ramos, S. R. R., Silva, A. V. C. da, & Lédo, C. A. da S. (2014). Mannitol for coconut ex situ conservation by minimum growth. Pesquisa Agropecuaria Brasileira, 49(2), 148-151. https://doi.org/10.1590/S1678-3921.pab2014.v49.17435