Híbridos somáticos assimétricos de citros produzidos pela fusão de protoplastos irradiados e tratados com iodoacetamida

Autores

  • Claudine Maria de Bona IAPAR
  • Jean Howe Gould Texas A&M University - TAMU
  • J. Creighton Miller Jr Texas A&M University - TAMU
  • David Stelly Texas A&M University - TAMU
  • Eliezer Silva Louzada Texas A&M UNiversity Kingsville - TAMUK

DOI:

https://doi.org/10.1590/S1678-3921.pab2009.v44.1832

Palavras-chave:

<i>Citrus</i> sp., irradiação gama, pomelo, fusão de protoplasto, hibridação somática, laranja doce

Resumo

O objetivo deste trabalho foi produzir híbridos somáticos assimétricos de citros pela fusão de protoplastos irradiados com raios gama e protoplastos tratados com iodoacetamida. Protoplastos foram isolados de suspensões celulares embriogênicas de pomelo (Citrus paradisi Macfad.), cultivares Ruby Red e Flame, de laranja doce (C. sinensis Osbeck) 'Itaboraí', 'Natal', Valencia' e 'Succari', de tangerinas 'Satsuma' (C. unshiu Marcow.) e 'Changsha' (C. reticulata Blanco) e de tangor 'Murcott' (C. reticulata x C. sinensis). Protoplastos doadores foram expostos a raios gama e protoplastos receptores foram tratados com 3 mmol L-1 de iodoacetamida (IOA) e fusionados para hibridação assimétrica. Embriões assimétricos foram germinados e as brotações produzidas foram enxertadas sobre porta-enxertos de sementes de 'Laranja azeda', 'Limão rugoso' ou citrumelo 'Swingle' (C. paradisi x Poncirus trifoliata) x 'Sunki' ou enraizadas após embebição em solução de -- (IBA) e aclimatadas em casa de vegetação. A análise de ploidia foi realizada por meio de citometria de fluxo e a hibridicidade foi confirmada por análise de polimorfismo de comprimento de fragmentos amplificados (AFLP) de amostras de DNA das plântulas. A melhor combinação doador-recipiente foi de protoplastos de 'Ruby Red' irradiados com 80 Gy, fusionados com protoplastos de 'Succari' tratados por 20 min com IOA. Plantas tetraploides e aneuploides foram produzidas e o problema de recalcitrância ao enraizamento foi resolvido pela introdução das bases das brotações em 3.000 mg L-1 de IBA por 10 min.

Biografia do Autor

Claudine Maria de Bona, IAPAR

Dr. Claudine Bona, a fruit researcher (pomology, horticulture) of the Agronomic Institute of Parana (IAPAR), Brazil, received her B.S. and M.S. in Agronomy and Plant Sciences from the Federal University of Parana in 1998 and 2002, respectively. She received her Ph.D. in Horticulture from Texas A&M University in 2007 and Post-Doctorate from the Federal University of Parana in 2008. Her research focuses on banana and citrus crop improvement.

Jean Howe Gould, Texas A&M University - TAMU

Adjunct Assistant and Adjunct Professor of the Department of Horticultural Sciences, Jean Gould received her B.A. in Liberal Arts & Sciences from the University of Illinois, Champaign-Urbana and her Ph.D. in Plant Physiology from the University of California, Riverside. Dr. Gould's area of research is genetic engineering and biotechnology.

J. Creighton Miller Jr, Texas A&M University - TAMU

Professor, Department of Horticultural Sciences, Dr. Creighton Miller received his B.S. and M.S. in General Studies and Horticulture from Louisiana State University in 1965 and 1967, respectively. He received his Ph.D. in Horticulture (Breeding and Genetics) from Michigan State University in 1972. Dr. Miller is a Member of the Graduate Faculties of Horticulture, Genetics, and Molecular and Environmental Plant Sciences at Texas A&M University.

David Stelly, Texas A&M University - TAMU

Professor of Plant Breeding, Genetics, Molecular and Environmental Plant Sciences, Dr. Stelly received his B.S. in Genetics from the University of Wisconsin-Madison in 1975, his M.S. in Plant Breeding and Cytogenetics from Iowa State University in 1979 and his Ph.D. in Plant Breeding and Plant Genetics from the University of Wisconsin-Madison in 1983. His research addresses basic and practical needs related to plant reproductive genetics or biology, evolution, chromosome biology and cytogenetic manipulation, genomics, genetic barriers to introgression, or other processes that impact our ability to genetically analyze, manipulate and improve plants.

Eliezer Silva Louzada, Texas A&M UNiversity Kingsville - TAMUK

Assistant Professor of the Citrus Center, at Texas A&M University-Kingsville, in Weslaco, Dr. Louzada is an Adjunct Member of the Graduate Faculty of TAMU. He received his B.S. degree in Agronomy and his M.S. degree in Soil Science from the Federal Rural University of Rio de Janeiro, Brazil in 1975 and 1978. He received his Ph.D. in Genetics in a joint program between the Federal University of Rio de Janeiro and The University of Florida in 1993. Dr. Louzada's research is divided in two areas, breeding, and horticulture. The main focus of his breeding research is to develop a new methodology to allow the transference of a single chromosome between citrus species, between citrus wild relatives and citrus, and between citrus and a short cycle plant.

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Publicado

2010-11-18

Como Citar

Bona, C. M. de, Gould, J. H., Jr, J. C. M., Stelly, D., & Louzada, E. S. (2010). Híbridos somáticos assimétricos de citros produzidos pela fusão de protoplastos irradiados e tratados com iodoacetamida. Pesquisa Agropecuária Brasileira, 44(5), 454–462. https://doi.org/10.1590/S1678-3921.pab2009.v44.1832

Edição

v.44, n.5, maio 2009

Seção

FRUTICULTURA