Image analysis of sweet potato calluses in vitro

Authors

  • Amanda Lima Vilela Universidade Federal de Lavras, Departamento de Agricultura, Lavras, MG.
  • Filipe Almendagna Rodrigues Universidade Federal de Lavras, Departamento de Agricultura, Lavras, MG.
  • Raquel Maria de Oliveira Pires Universidade Federal de Lavras, Departamento de Agricultura, Lavras, MG.
  • Gracielle Vidal Silva Andrade Universidade Federal de Lavras, Departamento de Agricultura, Lavras, MG.
  • Joyce Dória Universidade Federal de Lavras, Departamento de Agricultura, Lavras, MG.
  • Moacir Pasqual Universidade Federal de Lavras, Departamento de Agricultura, Lavras, MG.

Keywords:

Ipomoea batatas, GroundEye, micropropagation, tissue culture

Abstract

The objective of this work was to evaluate the use of the GroundEye equipment to characterize calluses from sweet potato explants. Sweet potato calluses from different explants (leaf, stem, and root) were grown in a culture medium with four growth regulators (naphtaleneacetic acid, 6-benzylaminopurine, 2,4-dichlorophenoxyacetic acid, and kinetin) and under two cultivation conditions (light and dark). The GroundEye system was used to analyze callus geometry and color, capturing color variation efficiently across different samples. In addition, the measurement of the area by the software directly reflects callus mass. Therefore, GroundEye is efficient in evaluating the geometry and color of calluses from explants of the Brazlândia Branca sweet potato cultivar.

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Published

2025-09-15

How to Cite

Lima Vilela, A., Rodrigues, F. A., de Oliveira Pires, R. M., Vidal Silva Andrade, G., Dória, J., & Pasqual, M. (2025). Image analysis of sweet potato calluses in vitro. Pesquisa Agropecuaria Brasileira, e03961. Retrieved from https://apct.sede.embrapa.br/pab/article/view/28142

Issue

V.60, Jan./Dec., 2025: Continuos publication

Section

HORTICULTURAL SCIENCE