Identification of the begomovirus resistance genes Ty‑2 and Ty‑3 in tomato genotypes

Authors

  • Jorge González Aguilera Federal University of Viçosa image/svg+xml
  • Francisco Dueñas Hurtado Instituto Nacional de Ciencias Agrícolas image/svg+xml
  • Cesar Augusto Diniz Xavier Departamento de Fitopatologia
  • Bruno Soares Laurindo Departamento de Fitotecnia
  • Carlos Nick Departamento de Fitotecnia
  • Marta Álvarez Gil Instituto Nacional de Ciencias Agrícolas image/svg+xml
  • Derly José Henriques da Silva Departamento de Fitotecnia
  • Francisco Murilo Zerbini Departamento de Fitopatologia

DOI:

https://doi.org/10.1590/S1678-3921.pab2011.v46.9936

Keywords:

Solanum lycopersicum, Solanum peruvianum, geminivirus, PCR, genetic resources

Abstract

The objective of this work was to identify the presence of the genes begomovirus resistence Ty‑2 and Ty‑3 in tomato accessions of the Banco de Germoplasma de Hortaliças of the Universidade Federal de Viçosa. The oligonucleotides TO302 F/R and FLUW25 F/R were used in PCR analysis to verify the presence of markers associated to the genes Ty‑2 and Ty‑3, respectively. The Ty‑2 gene was observed in heterozygosis in the accession BGH-6881 (Solanum peruvianum), and the Ty‑3 gene in homozygosis in the accessions BGH-6878, BGH-6897 (S. lycopersicum), and in heterozygosis in acession BGH-6881. The identification of resistance genes, using PCR analysis, represents an advance for tomato breeding programs in Brazil.

Author Biographies

  • Jorge González Aguilera, Federal University of Viçosa
    http://lattes.cnpq.br/8562342815666974
  • Cesar Augusto Diniz Xavier, Departamento de Fitopatologia
    http://lattes.cnpq.br/9062524731229088
  • Bruno Soares Laurindo, Departamento de Fitotecnia
    http://lattes.cnpq.br/5267666881711305
  • Carlos Nick, Departamento de Fitotecnia
    http://lattes.cnpq.br/8999214264404271
  • Derly José Henriques da Silva, Departamento de Fitotecnia
    http://lattes.cnpq.br/6243238937742884
  • Francisco Murilo Zerbini, Departamento de Fitopatologia
    http://lattes.cnpq.br/6507826653693228

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Published

2011-09-02

Issue

v.46, n.7, jul. 2011

Section

SCIENTIFIC NOTES

How to Cite

Aguilera, J. G., Hurtado, F. D., Xavier, C. A. D., Laurindo, B. S., Nick, C., Gil, M. Á., da Silva, D. J. H., & Zerbini, F. M. (2011). Identification of the begomovirus resistance genes Ty‑2 and Ty‑3 in tomato genotypes. Pesquisa Agropecuaria Brasileira, 46(7), 772-775. https://doi.org/10.1590/S1678-3921.pab2011.v46.9936

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