Selection and molecular characterization of Bacillus thuringiensis isolates for controlling Spodoptera spp.

Authors

  • Kelly Christiane Constanski Universidade Estadual de Londrina, Centro de Ciências Agrárias
  • Janaina Zorzetti Universidade Estadual de Londrina, Centro de Ciências Agrárias
  • Gislayne Trindade Vilas Boas Universidade Estadual de Londrina, Centro de Ciências Agrárias
  • Ana Paula Scaramal Ricietto Universidade Estadual de Londrina, Centro de Ciências Agrárias
  • Fernanda Aparecida Pires Fazion Universidade Estadual de Londrina, Centro de Ciências Agrárias
  • Laurival Vilas Boas Universidade Estadual de Londrina, Centro de Ciências Agrárias
  • Rose Gomes Monnerat Embrapa Recursos Genéticos e Biotecnologia
  • Pedro Manuel Oliveira Janeiro Neves Universidade Estadual de Londrina, Centro de Ciências Agrárias

DOI:

https://doi.org/10.1590/S1678-3921.pab2015.v50.21171

Keywords:

Spodoptera eridania, Spodoptera frugiperda, biological control, cry genes, Cry proteins

Abstract

The objective of this work was to select and molecularly characterize Bacillus thuringiensis isolates toxic to Spodoptera eridania and S. frugiperda. Thirty four isolates were subjected to the bioassay, of which three were selected and used to estimate the LC50. The selected isolates did not differ from the HD‑1 standard line. In the molecular characterization, the presence of the cry1 and cry2 genes was identified in the BR37 and BR94 isolates, and of the cry4A, cry4B, cry10, cry11, and cyt1 genes in the BR58 isolate, which confirmed the obtained protein profile of 130, 70, and 65 kDa. Bipyramidal and spherical crystals were identified. The BR58 isolate, although not containing the genes related to toxicity to Lepidoptera, causes mortality in both species.

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Published

2015-09-03

How to Cite

Constanski, K. C., Zorzetti, J., Vilas Boas, G. T., Ricietto, A. P. S., Fazion, F. A. P., Vilas Boas, L., … Neves, P. M. O. J. (2015). Selection and molecular characterization of <i>Bacillus thuringiensis</i> isolates for controlling <i>Spodoptera</i> spp. Pesquisa Agropecuaria Brasileira, 50(8), 730–733. https://doi.org/10.1590/S1678-3921.pab2015.v50.21171

Issue

v.50, n.8, ago. 2015

Section

SCIENTIFIC NOTES