Polymorphisms in MyoD1, MyoG, MyF5, MyF6, and MSTN genes in Santa Inês sheep

Authors

  • Luis Paulo Batista Sousa Junior Universidade Federal da Bahia, Departamento de Zootecnia, Avenida Adhemar de Barros, no 500, Ondina, CEP 40170-110 Salvador, BA.
  • Ariana Nascimento Meira Universidade Federal da Bahia, Departamento de Zootecnia, Avenida Adhemar de Barros, no 500, Ondina, CEP 40170-110 Salvador, BA,
  • Hymerson Costa Azevedo Embrapa Tabuleiros Costeiros, Avenida Beira Mar, no 3250, Jardins, CEP 49025-040 Aracaju, SE.
  • Evandro Nevez Muniz Embrapa Tabuleiros Costeiros, Avenida Beira Mar, no 3250, Jardins, CEP 49025-040 Aracaju, SE.
  • Luiz Lehmann Coutinho Universidade de São Paulo, Avenida Pádua Dias, no 11, Agronomia, CEP 13635-900 Piracicaba, SP.
  • Gerson Barreto Mourão Universidade de São Paulo, Avenida Pádua Dias, no 11, Agronomia, CEP 13635-900 Piracicaba, SP.
  • Victor Breno Pedrosa Universidade Estadual de Ponta Grossa, Avenida General Carlos Cavalcanti, no 4.748, Uvaranas, CEP 84030-900 Ponta Grossa, PR.
  • Luís Fernando Batista Pinto Universidade Federal da Bahia, Departamento de Zootecnia, Avenida Adhemar de Barros, no 500, Ondina, CEP 40170-110 Salvador, BA.

DOI:

https://doi.org/10.1590/S1678-3921.pab2019.v54.26630

Keywords:

Ovis aries, candidate genes, frequencies, variants

Abstract

The objective of this work was to sequence the MyoD1, MyoG, MyF5, MyF6, and MSTN genes and to identify polymorphisms in Santa Inês sheep (Ovis aries). A total of 192 lambs with 240 days of age were evaluated, and these genes were sequenced to be compared with the reference sequence in the Ovis aries genome. Genotype and allele frequencies were estimated, and the Hardy-Weinberg equilibrium was tested. Fragments containing 2,493 bp (MyoD1), 1,836 bp (MyoG), 2,813 bp (MyF5), 1,126 bp (MyF6), and 2,380 bp (MSTN) were obtained, and, in these sequences, 160 variants were identified. These polymorphisms were distributed as follows: 59 (MyoD1), 24 (MyoG), 63 (MyF5), 4 (MyF6), and 10 (MSTN). One hundred and four were novel polymorphisms, 45 in MyoD1, 2 in MyoG, 56 in MyF5, and 1 in MSTN. Regarding site, 61 were in intron (27 in MyoD1, 16 in MyoG, 5 in MyF5, 3 in MyF6, and 10 in MSTN), 87 in coding region (22 in MyoD1, 8 in MyoG, 56 in MyF5, and 1 in MyF6), and 12 on 3’UTR (10 in MyoD1 and 2 in MyF5). Therefore, the MyoD family and MSTN genes have several polymorphisms in Santa Inês sheep, which can be useful for association studies.

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Published

2019-11-19

How to Cite

Sousa Junior, L. P. B., Meira, A. N., Azevedo, H. C., Muniz, E. N., Coutinho, L. L., Mourão, G. B., … Pinto, L. F. B. (2019). Polymorphisms in <i>MyoD1</i>, <i>MyoG</i>, <i>MyF5</i>, <i>MyF6</i>, and <i>MSTN</i> genes in Santa Inês sheep. Pesquisa Agropecuaria Brasileira, 54(X), e01132. https://doi.org/10.1590/S1678-3921.pab2019.v54.26630

Issue

V.54, Jan./Dec., 2019 :Continuos publication

Section

GENETICS