In vitro organogenesis from internodal segments of adult sweet orange plants

Authors

  • Meire Menezes Bassan Universidade de São Paulo/ESALQ
  • Francisco de Assis Alves Mourão Filho Universidade de São Paulo/ESALQ
  • Luzia Yuriko Miyata Universidade de São Paulo/ESALQ
  • Beatriz Madalena Januzzi Mendes USP

DOI:

https://doi.org/10.1590/S1678-3921.pab2011.v46.10055

Keywords:

Citrus sinensis, adventitious buds, biotechnology, plant growth regulators

Abstract

The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27ºC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valência was promoted by EME supplemented with BAP, while NAA showed no apparent effect.

Author Biographies

Meire Menezes Bassan, Universidade de São Paulo/ESALQ

http://lattes.cnpq.br/0890076311747304

Luzia Yuriko Miyata, Universidade de São Paulo/ESALQ

Departamento de Produção Vegetal

Aluna do PPG Fitotecnia (Doutorado)

Beatriz Madalena Januzzi Mendes, USP

http://lattes.cnpq.br/9469987954896496

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Published

2011-08-11

How to Cite

Bassan, M. M., Mourão Filho, F. de A. A., Miyata, L. Y., & Mendes, B. M. J. (2011). In vitro organogenesis from internodal segments of adult sweet orange plants. Pesquisa Agropecuaria Brasileira, 46(6), 672–674. https://doi.org/10.1590/S1678-3921.pab2011.v46.10055

Issue

v.46, n.6, jun. 2011

Section

SCIENTIFIC NOTES