Vitamin C and endogenous cortisol in foreign‑body inflammatory response in pacus

Authors

  • Marco Antonio de Andrade Belo Faculdade de Ciências Agrárias e Veterinárias – FCAV (UNESP)
  • Julieta Rodini Engrácia de Moraes Faculdade de Ciências Agrárias e Veterinárias – FCAV (UNESP)
  • Vando Edésio Soares Universidade Camilo Castelo Branco
  • Maurício Laterça Martins Universidade Federal de Santa Catarina
  • Camila Dantas Brum UNESP, Centro de Aquicultura
  • Flávio Ruas de Moraes Faculdade de Ciências Agrárias e Veterinárias – FCAV (UNESP)

DOI:

https://doi.org/10.1590/S1678-3921.pab2012.v47.10409

Keywords:

Piaractus mesopotamicus, ascorbic acid, glucocorticoid, inflammation, Langhans cells, multinucleated giant cells.

Abstract

The objective of this work was to evaluate the effect of food supplementation with vitamin C on macrophage and multinucleated giant cell (MGC) activities of pacus at two stocking densities. The experiment was carried out in a 2x2x3 split-plot factorial arrangement with: 0 and 500 mg kg‑1 vitamin C; 5 and 20 kg m‑3 stocking densities; and evaluation times at 3, 6, and 12 days after the subcutaneous implantation of glass coverslips (DPI). The number of macrophages and MGC, as well as cortisol and glucose plasma levels were determined. The number of macrophages and MGC with two to five nuclei was significantly greater in fish supplemented with vitamin C at 5 kg m‑3 stocking density at 3 DPI in comparison to nonsupplemented ones. The macrophage and MGC counts were lower in fish with high‑plasma cortisol concentration. Supplementation with 500 mg vitamin C benefits macrophage activity on foreign‑body inflammation, and high‑cortisol concentration has suppressive effects on this response.

Author Biographies

Marco Antonio de Andrade Belo, Faculdade de Ciências Agrárias e Veterinárias – FCAV (UNESP)

Departamento de Patologia Veterinária

Julieta Rodini Engrácia de Moraes, Faculdade de Ciências Agrárias e Veterinárias – FCAV (UNESP)

Departamento de Patologia Veterinária

Vando Edésio Soares, Universidade Camilo Castelo Branco

Campus de Descalvado

Maurício Laterça Martins, Universidade Federal de Santa Catarina

Departamento de Aquicultura

Camila Dantas Brum, UNESP, Centro de Aquicultura

Laboratório de Patologia de Organismos Aquáticos

Flávio Ruas de Moraes, Faculdade de Ciências Agrárias e Veterinárias – FCAV (UNESP)

Departamento de Patologia

 

Published

2012-08-13

How to Cite

Belo, M. A. de A., Moraes, J. R. E. de, Soares, V. E., Martins, M. L., Brum, C. D., & Moraes, F. R. de. (2012). Vitamin C and endogenous cortisol in foreign‑body inflammatory response in pacus. Pesquisa Agropecuaria Brasileira, 47(7), 1017–1023. https://doi.org/10.1590/S1678-3921.pab2012.v47.10409

Issue

Section

VETERINARY SCIENCE