Genetic variation among accessions of the genus Manihot by ISSR markers

Authors

  • Kaliny Veiga Pessoa da silva UFRPE
  • Alfredo Augusto da Cunha Alves Embrapa-Labex
  • Maria Isabel Gomes Martins UFRPE
  • Cláusio Antônio Ferreira de Melo Universidade Estadual de Santa Cruz
  • Reginaldo de Carvalho UFRPE

DOI:

https://doi.org/10.1590/S1678-3921.pab2011.v46.10850

Keywords:

germplasm, genetic diversity, cassava, polymorphism.

Abstract

The objective of this work was to evaluate the genetic diversity within and among accessions of Manihot by using ISSR markers. Five species and two varieties of Manihot, besides two species of the genus Croton, used as the out-group, were analyzed by using 20 oligonucleotide (Olii) ISSR UBC primers. To analyze the similarity index between species and accessions, the Jaccard and simple matching coefficients were used. The 20 Olii tested were highly polymorphic in all species analyzed, and 89.7% of the loci were polymorphic. A higher genetic variability is observed among different species of Manihot, such as M. dichotoma var. undulata and M. caerulescens, than among individuals of the same species, such as M. dichotoma and M. dichotoma var. undulata.

Author Biographies

Kaliny Veiga Pessoa da silva, UFRPE

http://lattes.cnpq.br/0579417795319098

Maria Isabel Gomes Martins, UFRPE

http://lattes.cnpq.br/8446164424520637

Cláusio Antônio Ferreira de Melo, Universidade Estadual de Santa Cruz

http://lattes.cnpq.br/4143311508589631

Reginaldo de Carvalho, UFRPE

http://lattes.cnpq.br/5208573547132664

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Published

2011-12-14

How to Cite

Pessoa da silva, K. V., Alves, A. A. da C., Martins, M. I. G., de Melo, C. A. F., & de Carvalho, R. (2011). Genetic variation among accessions of the genus <i>Manihot</i> by ISSR markers. Pesquisa Agropecuaria Brasileira, 46(9), 1082–1088. https://doi.org/10.1590/S1678-3921.pab2011.v46.10850

Issue

v.46, n.9, set. 2011

Section

GENETICS