Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee

Authors

  • Gabriella Santos Pereira Universidade Federal de Lavras, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brazil.
  • Lilian Padilha Embrapa Café, Parque Estação Biológica, s/no, CEP 70770-901 Brasília, DF, Brazil
  • Edila Vilela Resende Von Pinho Universidade Federal de Lavras, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brazil
  • Rita de Kássia Siqueira Teixeira Universidade Federal de Lavras, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brazil.
  • Carlos Henrique Siqueira de Carvalho Embrapa Café, Parque Estação Biológica, s/no, CEP 70770-901 Brasília, DF, Brazil.
  • Mirian Peres Maluf Embrapa Café, Parque Estação Biológica, s/no, CEP 70770-901 Brasília, DF, Brazil
  • Bruna Line de Carvalho Universidade Federal de Lavras, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brazil.

DOI:

https://doi.org/10.1590/S1678-3921.pab2011.v46.10935

Keywords:

Coffea arabica, Leucoptera coffeella, EST-SSR, genotypes

Abstract

The objective of this work was to analyze coffee (Coffea arabica) genotypes resistant to the coffee leaf miner (Leucoptera coffeella) using microsatellite markers. Sixty‑six loci were evaluated, of which 63 were obtained from the Brazilian Coffee Expressed Sequence Tag (EST) database. These loci were amplified in bulks of individuals from F5 rogenies of 'Siriema' (C. arabica x C. racemosa) resistant and susceptible to the insect, in eight samples of C. racemosa, and in a F6 population of 'Siriema' with 91 individuals segregating for resistance to the leaf miner. Polymorphisms were verified for two simple sequence repeat (SSR) loci in bulks of the susceptible progenies. The two polymorphic alleles were present in around 70% of the susceptible genotypes in F5 and in approximately 90% of the susceptible individuals in F6. However, the polymorphic EST‑SSR markers among populations contrasting for resistance to leaf miner were not correlated to the evaluated characteristics. SSR markers show inter‑ and intraspecific polymorphism in C. arabica and C. racemosa.

Author Biographies

Gabriella Santos Pereira, Universidade Federal de Lavras, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brazil.

http://lattes.cnpq.br/6719530634422129

Lilian Padilha, Embrapa Café, Parque Estação Biológica, s/no, CEP 70770-901 Brasília, DF, Brazil

http://lattes.cnpq.br/7181520913798304

Edila Vilela Resende Von Pinho, Universidade Federal de Lavras, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brazil

http://lattes.cnpq.br/3353181277947455

Rita de Kássia Siqueira Teixeira, Universidade Federal de Lavras, Caixa Postal 3037, CEP 37200-000 Lavras, MG, Brazil.

http://lattes.cnpq.br/9561793313190689

Carlos Henrique Siqueira de Carvalho, Embrapa Café, Parque Estação Biológica, s/no, CEP 70770-901 Brasília, DF, Brazil.

http://lattes.cnpq.br/9233226749992478

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Published

2012-02-10

How to Cite

Pereira, G. S., Padilha, L., Von Pinho, E. V. R., Teixeira, R. de K. S., de Carvalho, C. H. S., Maluf, M. P., & de Carvalho, B. L. (2012). Microsatellite markers in analysis of resistance to coffee leaf miner in Arabica coffee. Pesquisa Agropecuaria Brasileira, 46(12), 1650–1656. https://doi.org/10.1590/S1678-3921.pab2011.v46.10935

Issue

v.46, n.12, dez. 2011

Section

GENETICS