Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao
DOI:
https://doi.org/10.1590/S1678-3921.pab2008.v43.1109Keywords:
biotechnology, cacao, gas exchange, micropropagation, morphogenesisAbstract
The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specifi c. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.Downloads
Published
2008-10-31
How to Cite
Silva, T. Édson R. da, Cidade, L. C., Alvim, F. C., Cascardo, J. C. de M., & Costa, M. G. C. (2008). Somatic embryogenesis and plant regeneration in elite clones of <i>Theobroma cacao</i>. Pesquisa Agropecuaria Brasileira, 43(10), 1433–1436. https://doi.org/10.1590/S1678-3921.pab2008.v43.1109
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SCIENTIFIC NOTES