In vitro and in vivo antioxidant activity of soft coffee

Authors

  • Sheila Andrade Abrahão Universidade Federal de Lavras Campus s/n 37200-000 - Lavras, MG - Brasil
  • Rosemary Gualberto Fonseca Alvarenga Universidade Federal de Lavras Campus universitário 37200-000 - Lavras, MG - Brasil - Caixa-Postal: 37
  • Raimundo Vicente Sousa Universidade Federal de Lavras Campus Universitário 37200-000 - Lavras, MG - Brasil - Caixa-Postal: 37
  • Adriene Lima Universidade Federal de Lavras, 37200-000 - Lavras, MG - Brasil - Caixa-Postal: 3037

DOI:

https://doi.org/10.1590/S1678-3921.pab2012.v47.11554

Keywords:

Coffea arabica, type 2 diabetes mellitus, lipid peroxidation, free radicals

Abstract

The objective of this work was to determine the in vivo and in vitro antioxidant activity of soft
coffee beverages, before and after roasting. For the analysis of the antioxidant activity, the methods of free
radical sequestration (DPPH) and of the chelating activity of Fe2+ ions were used. In vivo tests were performed
with diabetic Zucker rats with metabolic syndrome and control Zucker rats. The animals received daily doses
of coffee drinks by gavage for 30 days. After treatment, lipid peroxidation was evaluated. Roasted coffee
samples had the highest percentage of free radical sequestration. Concentration in both green and roasted
coffee samples was similar to that of the Trolox standard. From the roasted samples, medium roast stood out
with greater Fe2+ ion chelating activity. Green coffees showed higher quelating power than the roasted ones.
The compounds in the extract decreased the lipid peroxidation in the liver and kidney which is common in
cases of diabetes and metabolic syndrome. Coffee has an antioxidant activity and protects the liver and kidneys
of animals from lipid peroxidation commonly present in type 2 diabetes mellitus and metabolic syndrome.

Author Biographies

Sheila Andrade Abrahão, Universidade Federal de Lavras Campus s/n 37200-000 - Lavras, MG - Brasil

h

Rosemary Gualberto Fonseca Alvarenga, Universidade Federal de Lavras Campus universitário 37200-000 - Lavras, MG - Brasil - Caixa-Postal: 37

http://lattes.cnpq.br/7458825786380786

Raimundo Vicente Sousa, Universidade Federal de Lavras Campus Universitário 37200-000 - Lavras, MG - Brasil - Caixa-Postal: 37

http://lattes.cnpq.br/9921619486331279

Adriene Lima, Universidade Federal de Lavras, 37200-000 - Lavras, MG - Brasil - Caixa-Postal: 3037

http://lattes.cnpq.br/6516064880031804

Downloads

Published

2012-03-22

How to Cite

Abrahão, S. A., Alvarenga, R. G. F., Sousa, R. V., & Lima, A. (2012). In vitro and in vivo antioxidant activity of soft coffee. Pesquisa Agropecuaria Brasileira, 47(1), 127–133. https://doi.org/10.1590/S1678-3921.pab2012.v47.11554

Issue

v.47, n.1, jan. 2012

Section

FOOD TECHNOLOGY