Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production

Authors

  • Cleusa Maria Mantovanello Lucon Instituto Biológico
  • Claudia Mitsue Koike
  • Alice Ishida Ishikawa
  • Flávia Rodrigues Alves Patrício
  • Ricardo Harakava

DOI:

https://doi.org/10.1590/S1678-3921.pab2009.v44.1194

Keywords:

<i>Cucumis sativus</i>, antagonists, biological control, selection method, damping-off

Abstract

The objective of this work was to select and identify Trichoderma spp. isolates for the control of Rhizoctonia solani (AG-4) damping-off on cucumber (Cucumis sativus L.) seedlings, as well as to evaluate the effects of increasing concentrations and different combinations of the most effi cient isolates in the disease control. The experiments were carried out in a greenhouse with 490 isolates. The disease on cucumber seedlings was evaluated one week after the application of a commercial substrate infested with both antagonist (1%) and pathogen (1%) to the seedlings’ root collar. The twelve isolates that conferred more than 85% of disease reduction were further evaluated in pathogen control (1%) at the concentrations 0.5, 1, 2, 3 and 4%. The effect of combining fi ve of the most promising isolates in disease control was also evaluated. The most effective isolates were identifi ed through the sequencing of the ribosomal internal transcribed spacers (ITS) region. Out of the 490 isolates tested 44 (9%) caused reduction of damping-off. Antagonist concentrations higher than 2% conferred the most effective disease control. Only two combinations of isolates resulted in increased disease control. The most effective isolates were identifi ed as T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) and T. asperellum (IB44). Three isolates could not be identifi ed at species level.

Author Biography

Cleusa Maria Mantovanello Lucon, Instituto Biológico

Bióloga, mestre em Microbiologia Aplicada pela ESALq/USP e doutora em Microbiologia Aplicada pela UNESP/Rio Claro-SP. Funcionária efetiva do Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal, no Instituto Biológico, desde 1992, no cargo de Pesquisador Científica, atualmente nível V

Published

2010-11-09

How to Cite

Lucon, C. M. M., Koike, C. M., Ishikawa, A. I., Patrício, F. R. A., & Harakava, R. (2010). Bioprospection of <i>Trichoderma</i> spp. isolates to control <i>Rhizoctonia solani</i> on cucumber seedling production. Pesquisa Agropecuaria Brasileira, 44(3), 225–232. https://doi.org/10.1590/S1678-3921.pab2009.v44.1194

Issue

Section

PHYTOPATHOLOGY