The inactivation of newcastle disease virus by Beta-Propiolactone, Acethyleneimine and binary Elhyleneimine

Abstract

The inactivating action of BPL, AEI and BEl upon the infectivity and hemagglutinating activity of Newcastle Disease virus (Macapá strain), was studied. The inactivant was addcd to infected allantoic fluid, incubated at 37 ºC and samples collected at appropriate intervals to determine infectivity, hemagglutination titer and sterility. BPL at a final concentration of 1:4000 revealed higher inactivation at two hours incubation period and complete inactivation by six hours and 16 minutes. AEI, at a final concentration of 0,05%, showed more accentuated inactivation around six hours incubation períod and complete inactivation calculated to occur by 11 hours and 28 minutes. The 2% of BEl inactivating action was higher at four hours incubation with complete inactivation taking place at four hours and 18 minutes. All three chemical substances showed first order inactivating action, with linear infectivity decrease and no residual active virus particle