The influence of culture media on the sporulation of Septoria glycines hemmi, causal agent of soybean brown spot

Abstract

This work was undertaken to obtain a culture medium for fast and abundant spore production of Septoria glycines. Iniatially, oat meal agal, PDA, Czapek agar, tomato extract agar, soybean leaf extract agar, com meal agar, com meal agar, Fries agar, Sabouraud dextrose agar, and wheat flour agar media were compared. In a second trial the effect of adding a filter paper disc on the top of solidified media was tested. In both experiments the greatest number of pycnidiospores were produced on Fries medium. With the exception of Fries agar medium, filter paper did not increase spore production.