Peroxidase and superoxide dismutase banding patterns of some peach genotypes

Abstract

Twenty different peach (Prunus persica (L.) Batsch) genotypes were used for the purpose of developing electrophoretic techniques in peach, identify some peach genotypes at the isoenzymatic level and to report patterns of some enzymatic systems with characteristics of agroindustrial interest. For isoenzyme analysis, homogenized leaves from the mid-part of the branches were used. Upon analysis of esterase, peroxidase (anodic and cathodic) and of superoxide dismustase zymograms, it was verified that only the isoenzymes of anodic peroxidase and of superoxide dismutase could be utilized in determining isoenzymatic patterns of the genotypes. All peach genotypes exhibited the same banding patterns both for anodic peroxidase and for superoxide dismutase, which made impossible their use in identification of the genotypes. In the discussion of the study the hypothesis is raised that this uniformity may be related to the system of reproduction in peach, to the genetic uniformity of the genotypes and to a possible genetic convergence and also to the methodology employed.