Characterization of the vip3A gene and toxicity of Vip3Aa50 protein to fall armyworm and velvetbean caterpillar

Authors

  • Camila Soares Figueiredo Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdades de Ciências Agrarias e Veterinarias
  • Suzana Cristina Marucci Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdades de Ciências Agrarias e Veterinarias
  • Renata Izabel Dozzi Tezza Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdades de Ciências Agrarias e Veterinarias
  • Manoel Victor Franco Lemos Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdades de Ciências Agrarias e Veterinarias
  • Janete Apparecida Desidério Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdades de Ciências Agrarias e Veterinarias.

DOI:

https://doi.org/10.1590/S1678-3921.pab2013.v48.15363

Keywords:

Anticarsia gemmatalis, Spodoptera frugiperda, biological control, heterologous expression, vegetative insecticidal protein

Abstract

The objective of this work was to characterize the vip3A gene of Bacillus thuringiensis and to evaluate the toxicity of Vip3Aa50 protein to the fall armyworm (Spodoptera frugiperda) and velvetbean caterpillar (Anticarsia gemmatalis) larvae. The gene vip3A was amplified by specific PCR primers, generating a 2,370‑bp fragment. This fragment was cloned into the pGEM‑T Easy vector, and then it was sequenced, subcloned into the pET‑28a (+)’s expression vector, and inserted into Escherichia coli BL21 (DE3) cells. The Vip3Aa50 protein expression was induced by isopropyl‑β‑D‑1‑thiogalactopyranoside (IPTG), visualized in SDS‑PAGE, and detected by Western blot. The toxicity bioassay showed a high activity of Vip3Aa50 protein against both velvetbean and fall armyworm neonate larvae, with LC50 at 20.3 and 79.6 ng cm-2 respectively. The vip3Aa50 gene, is a new gene of vip3A class.

Published

2013-12-02

How to Cite

Figueiredo, C. S., Marucci, S. C., Tezza, R. I. D., Lemos, M. V. F., & Desidério, J. A. (2013). Characterization of the <i>vip3A</i> gene and toxicity of Vip3Aa50 protein to fall armyworm and velvetbean caterpillar. Pesquisa Agropecuaria Brasileira, 48(9), 1220–1227. https://doi.org/10.1590/S1678-3921.pab2013.v48.15363

Issue

Section

DISEASES CONTROL