Semisynthetic culture media for Nomuraea rileyi massal production

Abstract

During 1983, four experiments were carried out at the Agricultural School/UFRGS, in Porto Alegre, RS, Brazil, in order to test semisynthetic culture media for massal spore production by the entomogenous fungus Nomuraea rileyi (Farlow) Samson. The carrot-malt-agar (CMA), oats-malt-agar (OMA), tomato malt-agar (TMA), potato-dextro-agar (modified PDA) and Sabouraud- -maltose-agar plus yeast extract (SMAY) culture media were associated for the Passo Fundo, Camaquã and Londrina isolates of N. rileyi. pH levels of the media, incubation time, plant extract and malt meal amount were studied and the effects on sporulation determined. Differences in the amount of sporulation were observed among the isolates. CMA and OMA were effective sporulation media for N. rileyi. pH levels near 7,0 enhanced growth of Passo Fundo and Camaqui isolates of the fungus. Afeter 14 and 17 days of incubation, OMA and CMA did not favour significant increases in the fungus sporulation. N. rileyi grew well on AMA and CMA. The best combination components of CMA for sporulation of Londrina isolate was as follows: 340 ml/l of carrot extract, 22 g/l of malt meal and 12 min of sterillzation.