Identification of SNPs for fatty acid content in soybean by the HRM technique

Authors

  • Maria Fernanda Antunes da Cruz Universidade Federal de Viçosa, Departamento de Biologia Geral
  • Rafael Delmond Bueno Universidade Federal de Viçosa, Departamento de Bioquímica e Biologia Molecular
  • Franciele Barros de Souza Universidade Federal de Viçosa, Departamento de Bioquímica e Biologia Molecular
  • Maurilio Alves Moreira Universidade Federal de Viçosa, Departamento de Bioquímica e Biologia Molecular
  • Everaldo Gonçalves de Barros Universidade Federal de Viçosa, Departamento de Biologia Geral

DOI:

https://doi.org/10.1590/S1678-3921.pab2013.v48.17670

Keywords:

genotyping, molecular marker, plant breeding, single nucleotide polymorphisms, marker assisted selection

Abstract

The objective of this work was to identify SNPs in genes associated with fatty acid content in soybean and to implement the high resolution melting (HRM) technique for SNP genotyping. HRM primers were designed to discriminate SNP alleles in two mapping populations (RILs and F2) and followed the expected pattern of segregation. The ABI gene SNPs were significantly associated with stearic acid content (R2 = 12.14), and the ones from the FAD3B gene, with oleic (R2 = 14.69) and linolenic acid (R2 = 10.62) contents. The technique of genotyping SNPs by HRM is efficient to discriminate genotype classes.

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Published

2014-03-18

How to Cite

Cruz, M. F. A. da, Bueno, R. D., Souza, F. B. de, Moreira, M. A., & Barros, E. G. de. (2014). Identification of SNPs for fatty acid content in soybean by the HRM technique. Pesquisa Agropecuaria Brasileira, 48(12), 1596–1600. https://doi.org/10.1590/S1678-3921.pab2013.v48.17670

Issue

v.48, n.12, dez. 2013

Section

SCIENTIFIC NOTES