Adaptation of fluorescent technique for genotyping with new microsatellite markers in common bean

Authors

  • Paula Rodrigues Oblessuc UNICAMP
  • Tatiana de Campos UNICAMP
  • Juliana Morine Kupper Cardoso Instituto Agronomico de Campinas
  • Danilo Augusto Sforça UNICAMP
  • Renata Moro Baroni Instituto Agronomico de Campinas
  • Anete Pereira de Souza UNICAMP
  • Luciana Lasry Benchimol Instituto Agronômico de Campinas

DOI:

https://doi.org/10.1590/S1678-3921.pab2009.v44.2011

Keywords:

<i>Phaseolus vulgaris</i>, fluorescence, molecular marker, multiplex analysis

Abstract

The objectives of this work were to adapt the fluorescent labeling (PCR) technique using M13 universal primer for genotyping purposes, and to present a new set of microsatellite markers for common bean (Phaseolus vulgaris L.). A large population (380 common bean lines) was used for microsatellite genotyping. PCR fluorescent labeling method showed to be very efficient for multiplex analysis, providing lower costs and saving time, thus increasing the quality of genotyping analysis. A new set of 50 microsatellites developed from an enriched library derived from cultivar IAC-UNA was presented. This study provides better tools for assisting common bean breeding programs.

Author Biographies

Paula Rodrigues Oblessuc, UNICAMP

Graduated on Biology at UNICAMP in 2006. Master Degree on Genetics and Molecular Biology, Plant Genetics and Breeding area, Biology Institute of UNICAMP. Experience with common bean genetic analysis at Agronomic Institute of Campinas, conducting studies of QTL mapping with molecular markers and disease resistance.

Tatiana de Campos, UNICAMP

Graduated on Biology at USP in 2004. Doctor Degree on Genetics and Molecular Biology, Plant Genetics and Breeding area, Biology Institute of UNICAMP. Work with common bean genetic mapping and molecular markers.

Juliana Morine Kupper Cardoso, Instituto Agronomico de Campinas

Graduated on Biology at PUC in 2006. Master Degree on Genetics, Plant Breeding and Biotechnology, Agronomic Institute of Campinas. Experience with common bean genetic diversity analysis and molecular markers.

Danilo Augusto Sforça, UNICAMP

Graduation on Biology at UNICAMP. Work with common bean breeding and genetic mapping with molecular markers.

Renata Moro Baroni, Instituto Agronomico de Campinas

Graduated on Biology at PUC in 2006. Master Degree on Genetics, Plant Breeding and Biotechnology, Agronomic Institute of Campinas. Work with common bean QTL mapping, disease resistance and molecular markers.

Anete Pereira de Souza, UNICAMP

Graduated on Agronomic Engineering at ESALQ / USP in 1984. PhD in Cellular and Molecular Biology, Universite de Paris XI (Paris-Sud) in 1992. Currently, she is professor of Department of Genetics and Evolution (UNICAMP), Plant Genetics area. Operates mainly in genetics and plant molecular biology, with emphasis on molecular genetic mapping, and analysis of genetic diversity, structure and expression of proteins related to the fitopathogenicity.

Luciana Lasry Benchimol, Instituto Agronômico de Campinas

Graduated on Biology at UNICAMP in 1993. Master and Doctor Degrees on Genetics and Molecular Biology, Plant Genetics and Breeding area, Biology Institute of UNICAMP, in 1996 and 2002, respectively. PhD studies (2004) with common bean microsatellite development at Molecular Biology and Genetic Engineering Center (CBMEG / UNICAMP) and Agronomic Institute of Campinas (IAC). Researcher of IAC, Department of Plant Genetic Resources, since 2005. Teaching, since 2006, on a postgraduate course in Tropical and Subtropical Agriculture, area of Genetics, Plant Breeding and Biotechnology of the IAC. Have experience in molecular markers applied to breeding with emphasis on the improvement of common bean, mapping genes linked to disease resistance, selection assisted by markers, characterization of germplasm and development of microsatellites.

Published

2010-11-18

How to Cite

Oblessuc, P. R., Campos, T. de, Cardoso, J. M. K., Sforça, D. A., Baroni, R. M., Souza, A. P. de, & Benchimol, L. L. (2010). Adaptation of fluorescent technique for genotyping with new microsatellite markers in common bean. Pesquisa Agropecuaria Brasileira, 44(6), 638–644. https://doi.org/10.1590/S1678-3921.pab2009.v44.2011

Issue

Section

SCIENTIFIC NOTES