Identification of the B, Q, and native Brazilian biotypes of the Bemisia tabaci species complex using Scar markers

Authors

  • Paulo Roberto Queiroz Instituto Mato-Grossense do Algodão/Centro Universitário de Brasília
  • Erica Soares Martins Instituto Mato‑Grossense do Algodão, Rua Engenheiro Edgard Prado Arze, no 1.777, Edifício Cloves Vettorato, 2o Andar, Quadra 03, Setor A, Centro Político Administrativo, CEP 78049‑015 Cuiabá, MT.
  • Nazaré Klautau Universidade de Brasília
  • Luzia Lima Embrapa Recursos Genéticos e Biotecnologia
  • Lilian Praça Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Avenida W5 Norte (Final), Caixa Postal 02373, CEP 70770‑917 Brasília, DF.
  • Rose Gomes Monnerat Embrapa Recursos Genéticos e Biotecnologia

DOI:

https://doi.org/10.1590/S1678-3921.pab2016.v51.20475

Keywords:

Aleyrodidae, commodities, field samples, molecular marker, sweet potato whitefly

Abstract

The objective of this work was to develop sequence‑characterized amplified region (Scar) markers to identify the B, Q, and native Brazilian biotypes of the sweet potato whitefly [Bemisia tabaci (Hemiptera: Aleyrodidae)]. Random amplified polymorphic DNA (RAPD) amplification products, exclusive to the B and Brazilian biotypes, were selected after the analysis of 12,000 samples, in order to design a specific Scar primer set. The BT‑B1 and BT‑B3 Scar markers, used to detect the B biotype, produced PCR fragments of 850 and 582 bp, respectively. The BT‑BR1 Scar marker, used to identify the Brazilian biotype, produced a PCR fragment of 700 bp. The Scar markers were tested against the Q biotype, and a flowchart was proposed to indicate the decision steps to use these primers, in order to correctly discriminate the biotypes. This procedure allowed to identify the biotypes that occur in field samples, such as the B biotype. The used set of primers allowed to discriminate the B, Q, and native Brazilian biotypes of B. tabaci. These primers can be successfully used to identify the B biotype of B. tabaci from field samples, showing only one specific biotype present in all cultures.

Author Biography

Erica Soares Martins, Instituto Mato‑Grossense do Algodão, Rua Engenheiro Edgard Prado Arze, no 1.777, Edifício Cloves Vettorato, 2o Andar, Quadra 03, Setor A, Centro Político Administrativo, CEP 78049‑015 Cuiabá, MT.

Instituto Mato-Grossense do Algodão/Faculdades Integradas Promove de Brasília

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Published

2016-08-03

How to Cite

Queiroz, P. R., Martins, E. S., Klautau, N., Lima, L., Praça, L., & Monnerat, R. G. (2016). Identification of the B, Q, and native Brazilian biotypes of the <i>Bemisia tabaci</i> species complex using Scar markers. Pesquisa Agropecuaria Brasileira, 51(5), 555–562. https://doi.org/10.1590/S1678-3921.pab2016.v51.20475

Issue

v.51, n.5, maio 2016: Número Temático Pesquisa, Desenvolvimento e Inovações em Face de Ameaças Sanitárias para a Agropecuária

Section

ENTOMOLOGY