Chemical sterilization of culture medium for anthurium in vitro culture

Abstract

The objective of this study was to evaluate the use of chlorine dioxide or of peracetic acid in the chemical sterilization of culture medium and in the development of anthurium (Anthurium andraeanum) plantlets cultured in vitro. Treatments consisted of chlorine dioxide and peracetic acid at 0.005 and 0.010%. The autoclaved medium was used as control. The anthurium plantlets were grown in elongation and rooting MS medium with the concentration of macronutrients reduced by half. The peracetic acid, in the concentrations used, was not effective in the sterilization of culture medium. The chlorine dioxide had 100% efficiency in the sterilization of culture medium and caused no phytotoxicity to plantlets.