Characterization of rue extract and its potential for controlling rice blast

Authors

  • Karinna Bannach Reis Universidade Federal de Goiás, Faculdade de Farmácia, Laboratório de Pesquisas de Produtos Naturais, Goiânia, GO
  • Marcio Vinicius de Carvalho Barros Côrtes Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO
  • Frederico Severino Martins Universidade de São Paulo, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, SP
  • Marta Cristina Corsi de Filippi Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO
  • José Realino de Paula Universidade Federal de Goiás, Faculdade de Farmácia, Laboratório de Pesquisas de Produtos Naturais, Goiânia, GO
  • Edemilson Cardoso da Conceição Universidade Federal de Goiás, Faculdade de Farmácia, Laboratório de Pesquisas de Produtos Naturais, Goiânia, GO

DOI:

https://doi.org/10.1590/S1678-3921.pab2015.v50.21451

Keywords:

Magnaporthe oryzae, Ruta graveolens, bergapten, psoralen

Abstract

The objective of this work was to purify and standardize the rue (Ruta graveolens) extract and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast severity by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.

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Published

2015-12-23

How to Cite

Reis, K. B., Côrtes, M. V. de C. B., Martins, F. S., Filippi, M. C. C. de, Paula, J. R. de, & Conceição, E. C. da. (2015). Characterization of rue extract and its potential for controlling rice blast. Pesquisa Agropecuaria Brasileira, 50(12), 1121–1130. https://doi.org/10.1590/S1678-3921.pab2015.v50.21451

Issue

v.50, n.12, dez. 2015

Section

PHYTOPATHOLOGY