Optimization of heterologous microsatellites in piracanjuba

Authors

  • Fausto Moreira da Silva Carmo Universidade Federal de São João Del‑Rei, Departamento de Zootecnia, Laboratório de Recursos Genéticos, São João Del‑Rei, MG
  • Érico Macedo Polo Universidade Federal de São João Del‑Rei, Departamento de Zootecnia, Laboratório de Recursos Genéticos, São João Del‑Rei, MG
  • Marcos Antônio da Silva Universidade Federal de São João Del‑Rei, Departamento de Zootecnia, Laboratório de Recursos Genéticos, São João Del‑Rei, MG
  • Gabriel de Menezes Yazbeck Universidade Federal de São João Del‑Rei, Departamento de Zootecnia, Laboratório de Recursos Genéticos, São João Del‑Rei, MG

DOI:

https://doi.org/10.1590/S1678-3921.pab2015.v50.22109

Keywords:

Brycon orbignyanus, aquaculture, hatchery, molecular markers

Abstract

The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty‑one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh16) are valid to be applied in the study of piracanjuba.

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Published

2015-12-23

How to Cite

Carmo, F. M. da S., Polo, Érico M., Silva, M. A. da, & Yazbeck, G. de M. (2015). Optimization of heterologous microsatellites in piracanjuba. Pesquisa Agropecuaria Brasileira, 50(12), 1236–1239. https://doi.org/10.1590/S1678-3921.pab2015.v50.22109

Issue

v.50, n.12, dez. 2015

Section

SCIENTIFIC NOTES