Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids

Authors

  • Fernanda Motta da Costa Santos Universidade Federal de Lavras, Departamento de Biologia, Caixa Postal 3037, CEP 372000‑000 Lavras, MG.
  • Giovana Augusta Torres Universidade Federal de Lavras, Departamento de Biologia, Caixa Postal 3037, CEP 372000‑000 Lavras, MG.
  • Vânia Helena Techio Universidade Federal de Lavras, Departamento de Biologia, Caixa Postal 3037, CEP 372000‑000 Lavras, MG.
  • Antônio Vander Pereira Embrapa Gado de Leite, Rua Eugênio do Nascimento, no 610, Dom Bosco, CEP 36038-330 Juiz de Fora, MG.
  • Lisete Chamma Davide Universidade Federal de Lavras, Departamento de Biologia, Caixa Postal 3037, CEP 372000‑000 Lavras, MG.

DOI:

https://doi.org/10.1590/S1678-3921.pab2017.v52.24975

Keywords:

Pennisetum glaucum, Pennisetum purpureum, in situ hybridization, intergenomic pairing, interspecific hybridization

Abstract

The objective of this work was to evaluate, by genomic in situ hybridization (GISH), pairing configurations as potential indicators of recombination between chromosomes of different parental genomes, in two interspecific hybrids (elephant grass x pearl millet) artificially polyploidized. Anthers from young flower buds were used in the chromosomal preparations. The genomic probe was prepared with pearl millet DNA and labeled with biotin-16-dUTP by the nick translation reaction. Blocking DNA was prepared with genomic elephant grass DNA. The homoeologous intergenomic pairing, observed in the two hybrids, indicates the possibility of recombination between chromosomes of the parental genomes.

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Published

2017-10-11

How to Cite

Santos, F. M. da C., Torres, G. A., Techio, V. H., Pereira, A. V., & Davide, L. C. (2017). Intra- and intergenomic chromosomal pairing in artificially polyploidized elephant grass and pearl millet hybrids. Pesquisa Agropecuaria Brasileira, 52(9), 814–817. https://doi.org/10.1590/S1678-3921.pab2017.v52.24975

Issue

v.52, n.9, set. 2017

Section

SCIENTIFIC NOTES