Solidifying agents and activated charcoal for in vitro culture of Solanum sessiliflorum

Authors

  • Filipe Almendagna Rodrigues Universidade Federal de Lavras, Departamento de Agricultura, Laboratório de Cultura de Tecidos Vegetais, Campus Universitário, Caixa Postal 3037, CEP 37200-000 Lavras, MG.
  • Renata Alves Lara Silva Rezende Universidade Federal de Lavras, Departamento de Agricultura, Laboratório de Cultura de Tecidos Vegetais, Campus Universitário, Caixa Postal 3037, CEP 37200-000 Lavras, MG.
  • Moacir Pasqual Universidade Federal de Lavras, Departamento de Agricultura, Laboratório de Cultura de Tecidos Vegetais, Campus Universitário, Caixa Postal 3037, CEP 37200-000 Lavras, MG.
  • Maria Teresa Gomes Lopes Universidade Federal do Amazonas, Faculdade de Ciências Agrárias, Departamento de Produção Animal e Vegetal, Avenida General Rodrigo Otávio Jordão Ramos, no 1.200, Coroado I, CEP 69067-005 Manaus, AM

DOI:

https://doi.org/10.1590/S1678-3921.pab2017.v52.25186

Keywords:

maná cubiu, micropropagation, plant tissue culture, phytagel, Solanaceae

Abstract

The objective of this work was to evaluate the effects of the solidifying agents agar and phytagel and of activated charcoal on the in vitro cultivation of two maná cubiu (Solanum sessiliflorum) varieties: Thaís and Santa Luzia. The phytotechnical characteristics analyzed included number of leaves, number of roots, shoot and root length, and fresh matter of shoot and root. Regardless of the variety, phytagel was superior to agar as a culture medium. A greater number of leaves and longer shoots were observed in the Santa Luzia variety, in the absence of charcoal. The Thaís variety showed longer shoots and roots in the presence of charcoal.

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Published

2017-12-18

How to Cite

Rodrigues, F. A., Rezende, R. A. L. S., Pasqual, M., & Lopes, M. T. G. (2017). Solidifying agents and activated charcoal for in vitro culture of <i>Solanum sessiliflorum</i>. Pesquisa Agropecuaria Brasileira, 52(11), 1123–1126. https://doi.org/10.1590/S1678-3921.pab2017.v52.25186

Issue

v.52, n.11, nov. 2017

Section

SCIENTIFIC NOTES