Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut

Authors

  • Ana da Silva Lédo Embrapa Tabuleiros Costeiros, Avenida Beira Mar, no 3.250, Jardins, CEP 49025-040 Aracaju, SE.
  • Maria Jenderek ARS/USDA
  • Dianne Skogerboe United States Department of Agriculture, Agricultural Research Service, 1111 South Mason Street, 80526 Fort Collins, CO, USA.
  • Elise Staats ARS/USDA
  • Caroline de Araújo Machado Universidade Federal de Sergipe, Campus São Cristovão, Cidade Universitária Prof. José Aloísio de Campos, Avenida Marechal Rondon, s/no, Jardim Rosa Elze, CEP 49100-000 São Cristóvão, SE.
  • Leila Albuquerque Resende de Oliveira Universidade Federal de Sergipe

DOI:

https://doi.org/10.1590/S1678-3921.pab2018.v53.25585

Keywords:

Cocos nucifera, ex situ conservation, PVS3

Abstract

The objective of this work was to evaluate rewarming procedures and recovery media for Brazilian Green Dwarf coconut (Cocos nucifera) embryos cryopreserved by vitrification. The rewarming procedures evaluated were: T1, water bath at 38±2°C; and T2, unloading solution consisting of Y3 medium + 1.2 mol L-1 sucrose. The recovery media assessed were: M1, Y3 medium + 45 g L-1 sucrose + 1 mg L-1 gibberellic acid + 1 g L-1 activated charcoal + 2.2 g L-1 Gelrite; and M2, Y3 medium + 60 g L-1 sucrose + 2.2 g L-1 Gelrite. Rewarming procedures showed no significant effect, and the M1 media induced 72.5% regeneration.

Author Biography

Ana da Silva Lédo, Embrapa Tabuleiros Costeiros, Avenida Beira Mar, no 3.250, Jardins, CEP 49025-040 Aracaju, SE.

http://lattes.cnpq.br/8253115718345940 Aracaju, SE

Downloads

Additional Files

Published

2018-06-19

How to Cite

Lédo, A. da S., Jenderek, M., Skogerboe, D., Staats, E., Machado, C. de A., & Oliveira, L. A. R. de. (2018). Cryopreservation of zygotic embryos of the Brazilian Green Dwarf coconut. Pesquisa Agropecuaria Brasileira, 53(4), 514–517. https://doi.org/10.1590/S1678-3921.pab2018.v53.25585

Issue

v.53, n.4, abr. 2018

Section

SCIENTIFIC NOTES