Lytic bacteriophages as a potential alternative to control Staphylococcus aureus

Authors

  • Juliana Almeida Leite Instituto Oswaldo Cruz, Laboratório de Vírus Respiratório e do Sarampo, Avenida Brasil, no 4.365, Manguinhos, CEP 21040-360 Rio de Janeiro, RJ.
  • Hyago Passe Pereira Universidade Federal de Juiz de Fora, Rua José Lourenço Kelmer, s/no, Campus Universitário, São Pedro, CEP 36036-900 Juiz de Fora, MG.
  • Cristiano Amâncio Vieira Borges Embrapa Gado de Leite, Rua Eugênio do Nascimento, no 610, Dom Bosco, CEP 36038-330 Juiz de Fora, MG
  • Bruna Rios Coelho Alves Embrapa Gado de Leite, Rua Eugênio do Nascimento, no 610, Dom Bosco, CEP 36038-330 Juiz de Fora, MG.
  • Alessandra Isis Alves Pinheiro Ramos Embrapa Gado de Leite, Rua Eugênio do Nascimento, no 610, Dom Bosco, CEP 36038-330 Juiz de Fora, MG.
  • Marta Fonseca Martins Embrapa Gado de Leite, Rua Eugênio do Nascimento, no 610, Dom Bosco, CEP 36038-330 Juiz de Fora, MG.
  • Edna Froeder Arcuri Embrapa Gado de Leite, Rua Eugênio do Nascimento, no 610, Dom Bosco, CEP 36038-330 Juiz de Fora, MG.

DOI:

https://doi.org/10.1590/S1678-3921.pab2019.v54.26611

Keywords:

Staphylococcus aureus, biological control, endolysin, phage

Abstract

The objective of this work was to characterize autochthonous bacteriophages and to determine their lytic activity on Staphylococcus aureus. Six phages were isolated from dairy barn flush water through enrichment cultures with three S. aureus strains. All phages were characterized by DNA digestion by restriction enzymes and sequencing of the DNA fragment encoding endolysin. Each phage was tested against 100 S. aureus strains isolated from bovine mastitis and from dairy products using the lysis-plate method. The sequences of the endolysin gene were highly conserved, with nucleotide similarity higher than 99% among the isolated phages. Three domains involved in the recognition and lysis of the bacterial cell wall were identified. Two bacteriophages isolated from the dairy barns present high lytic activity on S. aureus, on a wide range of host strains, indicating their potential for studies on phage therapy in dairy cattle or as a biological control agent for dairy products.

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Published

2019-11-06

How to Cite

Leite, J. A., Pereira, H. P., Borges, C. A. V., Alves, B. R. C., Ramos, A. I. A. P., Martins, M. F., & Arcuri, E. F. (2019). Lytic bacteriophages as a potential alternative to control <i>Staphylococcus aureus</i>. Pesquisa Agropecuaria Brasileira, 54(X), e00917. https://doi.org/10.1590/S1678-3921.pab2019.v54.26611