Regeneration of pitaya by indirect organogenesis evaluated by scanning electron microscopy and flow cytometry

Authors

  • Mariane Aparecida Rodrigues Universidade Federal de Lavras, Departamento de Agricultura, Caixa Postal 3037, CEP 37200-900 Lavras, MG.
  • Flávia Aparecida da Silveira Universidade Federal de Lavras, Departamento de Agricultura, Caixa Postal 3037, CEP 37200-900 Lavras, MG.
  • Renata Amato Moreira Universidade Federal de Lavras, Departamento de Agricultura, Caixa Postal 3037, CEP 37200-900 Lavras, MG.
  • Marlúcia Souza Pádua Universidade Estadual de Minas Gerais, Avenida Paraná, no 3.001, Jardim Belvedere I, CEP 35501-170 Divinópolis, MG.
  • José Eduardo Brasil Pereira Pinto Universidade Federal de Lavras, Departamento de Agricultura, Caixa Postal 3037, CEP 37200-900 Lavras, MG.
  • Leila Aparecida Salles Pio Universidade Federal de Lavras, Departamento de Agricultura, Caixa Postal 3037, CEP 37200-900 Lavras, MG.
  • Dalilhia Nazaré dos Santos Empresa Mato-Grossense de Pesquisa, Assistência e Extensão Rural, Laboratório de Biotecnologia, Avenida Miguel Sutil, Distrito Dom Aquino, CEP 78015-100 Várzea Grande, MT.
  • Júlio Sílvio de Sousa Bueno Filho Universidade Federal de Lavras, Departamento de Ciências Exatas, Caixa Postal 3037, CEP 37200-900 Lavras, MG.
  • Letícia Alves Carvalho Reis University of Guelph, Department of Plant Agriculture, Crop Science Building, 50 Stone Road East, N1G 2W1 Guelph, ON.

DOI:

https://doi.org/10.1590/S1678-3921.pab2022.v57.27096

Keywords:

Hylocereus undatus, dragon fruit, endoreduplication, micropropagation

Abstract

The objective of this work was to evaluate the induction of indirect organogenesis by concentrations of dichlorophenoxyacetic acid (2,4-D)
and thidiazuron (TDZ) in pitaya (Hylocereus undatus) explants, using scanning electron microscopy and the flow cytometry technique. The treatments consisted of the concentrations of 0, 2.0, and 4.0 mg L-1 2,4-D and TDZ and of the combinations of these regulators. Percentages of callus coverage at 45 and 60 days were evaluated. The explants subjected to the treatments were analized by flow cytometry and scanning electron microscopy. All treatments induced endoreduplication, and there was no somaclonal variation. Under the combination of 2.0 mg L-1 TDZ and 4.0 mg L-1 2,4-D, calluses were formed in 95% of the explants, but were smaller than those produced with 2,4-D separately. The concentration of 2.0 mg L-1 TDZ induces the indirect organogenesis in pitaya explants, confirmed by the presence of conducting vessels through scanning electron microscopy.

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Published

2022-07-26

How to Cite

Rodrigues, M. A., Silveira, F. A. da, Moreira, R. A., Pádua, M. S., Pinto, J. E. B. P., Pio, L. A. S., … Reis, L. A. C. (2022). Regeneration of pitaya by indirect organogenesis evaluated by scanning electron microscopy and flow cytometry. Pesquisa Agropecuaria Brasileira, 57(Z), e02312. https://doi.org/10.1590/S1678-3921.pab2022.v57.27096

Issue

V.57, Jan./Dec., 2022 :Continuos publication

Section

POMOLOGY