In vitro viability of genipap pollen grains in different culture media

Authors

  • Gilmara da Silva Freire Universidade Federal de Sergipe, Cidade Universitária Prof. José Aloísio de Campos, Avenida Marechal Rondon, s/no, Jardim Rosa Elze, CEP 49100-000 São Cristovão, SE.
  • Letícia Bispo da Rocha Universidade Federal de Sergipe, Cidade Universitária Prof. José Aloísio de Campos, Avenida Marechal Rondon, s/no, Jardim Rosa Elze, CEP 49100-000 São Cristovão, SE.
  • Caroline de Araújo Machado Secretaria de Estado da Educação, do Esporte e da Cultura, Rua Gutemberg Chagas, no 169, Dia, CEP 49040-780 Aracaju, SE.
  • Ana Veruska Cruz da Silva Embrapa Tabuleiros Costeiros, Avenida Governador Paulo Barreto de Menezes (Beira Mar), no 3250, Jardins, CEP 49025-040 Aracaju, SE.
  • Ana da Silva Ledo Embrapa Tabuleiros Costeiros, Avenida Governador Paulo Barreto de Menezes (Beira Mar), no 3250, Jardins, CEP 49025-040 Aracaju, SE.

Keywords:

Genipa americana, in vitro pollen germination, plant breeding, pollen tube.

Abstract

The objective of this work was to evaluate the viability of genipap (Genipa americana) pollen grain at room temperature in different culture media. The experimental design was completely randomized in a 3x11 factorial arrangement (culture media x incubation times) with four replicates. The number of germinated pollen grains was analyzed at 24-hour intervals up to 288 hours after incubation at room temperature. The culture medium with 100 mg L-1 H3BO3, 80 g L-1 sucrose, and 1.0 g L-1 agar results in a higher to intermediate germination percentage, being the most suitable for studies on the in vitro viability of genipap pollen grains.

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Published

2022-10-25

How to Cite

Freire, G. da S., Rocha, L. B. da, Machado, C. de A., Silva, A. V. C. da, & Ledo, A. da S. (2022). In vitro viability of genipap pollen grains in different culture media. Pesquisa Agropecuaria Brasileira, 57(Z), e03020. Retrieved from https://apct.sede.embrapa.br/pab/article/view/27147

Issue

V.57, Jan./Dec., 2022 :Continuos publication

Section

POMOLOGY