In vitro organogenesis as an efficient method for the propagation of Dalbergia nigra
Keywords:
Dalbergia nigra, Brazilian rosewood, explants, in vitro culture, 6-benzylaminopurine, thidiazuronAbstract
The objective of this work was to establish an efficient protocol for the in vitro organogenesis of Dalbergia nigra. For this purpose, 30-day-old seedlings were sectioned at their cotyledonary nodes and nodal segments. These materials were cultivated in a medium with different combinations of the 6-benzylaminopurine and thidiazuron cytokinins. After 60 days in a growth chamber set at 27°C and a 16-hour photoperiod, growth characteristics were analyzed. Explants from the cotyledonary nodes show a greater morphogenetic potential, regardless of the addition of cytokinins. Cotyledonary nodes in the medium supplemented with 2.22 µmol L-1 BAP show the best combination for the in vitro propagation of D. nigra.
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Published
2022-12-05
How to Cite
Simões, I. M., Araujo, C. P. de, Mello, T. de, Rosa, T. L. M., Oliveira, N. V. de, Caldeira, M. V. W., … Alexandre, R. S. (2022). In vitro organogenesis as an efficient method for the propagation of <i>Dalbergia nigra</i>. Pesquisa Agropecuaria Brasileira, 57(Z), e02766. Retrieved from https://apct.sede.embrapa.br/pab/article/view/27165
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FORESTRY