Cryopreservation of Adenium obesum seeds in liquid nitrogen

Authors

  • Talita Angélica de Oliveira Rosa Universidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR.
  • Sérgio Pedro Junior Universidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR.
  • Débora Perdigão Tejo Universidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR.
  • Jean Carlo Baudraz de Paula Universidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR.
  • Gabriel Danilo Shimizu Universidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR.
  • Ricardo Tadeu de Faria Universidade Estadual de Londrina, Departamento de Agronomia, Rodovia Celso Garcia Cid, PR 445, Km 380, Campus Universitário, CEP 86057-970 Londrina, PR.

Keywords:

Adenium obesum, cryoprotectants, freezing tolerance, seed germination

Abstract

The objective of this work was to evaluate cryoprotectants on the physiological quality of Adenium obesum seeds preserved in liquid nitrogen. The following treatments were used: T1, control, without cryoprotectant and without immersion in liquid nitrogen; T2, with immersion in liquid nitrogen and without cryoprotectant; T3, with 0. 4 mol L-1 sucrose; T4, with 0.8 mol L-1 sucrose; T5, with 1.0 mol L-1 glycerol; T6, with 2.0 mol L-1 glycerol with PVS1; T7, with PVS1; T8, with PVS2; T9, with PVS2 + phloroglucinol (modified PVS2); and T10, with PVS3. Adenium obesum seeds show tolerance to immersion and storage in liquid nitrogen and do not need cryoprotectants to maintain their physiological quality when stored in a water content of 7.5%.

Downloads

Published

2024-08-22

How to Cite

Rosa, T. A. de O., Pedro Junior, S., Tejo, D. P., Paula, J. C. B. de, Shimizu, G. D., & de Faria, R. T. (2024). Cryopreservation of <i>Adenium obesum</i> seeds in liquid nitrogen. Pesquisa Agropecuaria Brasileira, 59(AB), e03442. Retrieved from https://apct.sede.embrapa.br/pab/article/view/27723

Issue

V.59, Jan./Dec., 2024 :Continuos publication

Section

SEED TECHNOLOGY