Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus

Authors

  • Claudine Maria de Bona Agricultural Research Institute of Parana (IAPAR)
  • David Stelly Texas A&M University (TAMU)
  • J. Creighton Miller Junior Texas A&M University (TAMU)
  • Eliezer Silva Louzada Texas A&M University - Kingsville (TAMUK)

DOI:

https://doi.org/10.1590/S1678-3921.pab2009.v44.3024

Keywords:

<i>Citrus, Swinglea glutinosa</i>, asymmetric somatic hybridization, gamma rays, hybrid cell lines, radiation hybrid mapping

Abstract

The objective of this work was to combine asymmetric somatic hybridization (donor-recipient fusion or gamma fusion) to microprotoplast-mediated chromosome transfer, as a tool to be used for chromosome mapping in Citrus. Swinglea glutinosa microprotoplasts were irradiated either with 50, 70, 100 or 200 gamma rays and fused to cv. Ruby Red grapefruit or Murcott tangor protoplasts. Cell colonies were successfully formed and AFLP analyses confirmed presence of S. glutinosa in both 'Murcott' tangor and 'Ruby Red' grapefruit genomes.

Author Biographies

Claudine Maria de Bona, Agricultural Research Institute of Parana (IAPAR)

B.S. in Agronomy, M.S. in Plant Sciences from Federal University of Parana (UFPR), Brazil, Ph.D. in Horticulture (Agronomy) from Texas A&M University. Post-doctoral (UFPR-CNPq).

Fruit Researcher - IAPAR.

David Stelly, Texas A&M University (TAMU)

Ph.D., Plant Breeding and Plant Genetics, University of Wisconsin-Madison, M.Sc., Plant Breeding and Cytogenetics, Iowa State University, B.Sc., Genetics, University of Wisconsin-Madison.

Director of the TAMU Laboratory for Plant Molecular Cytogenetics.

Member of the Crop Science Society of America, annual workshop organizer of the Plant & Animal Genome Conference, organizer of the First International Symposium on Apomixis, Award winner of the 1995 Cotton Genetics Research, and editorial of Chromosome Research, Korea Genetics, Crop Science, Genome, Journal of Heredity, and Euphytica.

J. Creighton Miller Junior, Texas A&M University (TAMU)

B.S. and M.S. in General Studies and Horticulture from Louisiana State University, Ph.D. in Horticulture (Breeding and Genetics) from Michigan State University.

Member of the Graduate Faculties of Horticulture, Genetics, and Molecular and Environmental Plant Sciences at TAMU.

Eliezer Silva Louzada, Texas A&M University - Kingsville (TAMUK)

B.S. degree in Agronomy and M.S. degree in Soil Science - Federal Rural University of Rio de Janeiro, Brazil in and Ph.D. degree in Genetics - University of Florida.

Adjunct Member of the Graduate Faculty

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Published

2010-12-22

How to Cite

Bona, C. M. de, Stelly, D., Junior, J. C. M., & Louzada, E. S. (2010). Fusion of protoplasts with irradiated microprotoplasts as a tool for radiation hybrid panel in citrus. Pesquisa Agropecuaria Brasileira, 44(12), 1616–1623. https://doi.org/10.1590/S1678-3921.pab2009.v44.3024

Issue

v.44, n.12, dez. 2009

Section

POMOLOGY