Effect of benzylamino purine and naphtahaleneacetic acid on shoot proliferation and growth In vitro of Coffea arabica L.

Abstract

Tissue culture technique is an important instrument for genetic plant breeding and plant propagation. Shoots with nearly 3 mm, excised from seedlings obtained in vitro of Coffea arabica L. Cv. catuaí, line LC-2077-2-5-44, were inoculated on medium composed of salts of Murashige and Skoog, vitamins of Morel, myoinositol (100 mg.l^-1), glycine (2 g.l^-1), sucrose (30 g.1^-1) and agar (7 g.1^-1), additioned by benzylamino purine (BAP) (0, 500, 1.000, 2.000, 2.500, 3.000, 3.500 and 4.000 mg.l^-1) and naphthaleneacetic acid (NAA) (0 and 200 mg.l^-1). The experiment was carried out under light (16 hours photoperiod) and 27^oC. The total bud multiplication was best on the medium with BAP 3.000 mg.l^-1 without NAA, after 90 days of culture. The best proliferation of shoots bigger than 1 cm took place in BAP 500 mg.l^-1 concentrations. The use of NAA 200 mg.l^-1 caused damage to shoot proliferation.