Plant regeneration in callus culture of brazilian wheat genotypes

Abstract

Sixteen wheat genotypes were evaluated for in vitro plant regeneration. Im­mature wheat embryos were inoculated on MS medium with 5 mg and 2 mg/1 2,4D to callus initiation. The rest of culture was done on MS medium with 0,5 mg/1, 0,2 mg/1 and without 2,4D in the media. The metodology used was suitable to regenerate plants of different Brazi­lian wheat genotypes. Variability among genotypes was observed to plant regeneration. Re­generative capacity changed, however, with different levels of 2,4D. The offspring of Nobre and Maringá genotypes from in vitro regenerated plants was evaluated on field to plant hei­ght, heading date and spikelet number and seed number per spike. Somaclonal variation was observed in both genotypes to all evaluated traits.