Vegetative propagation of coconut palm through in vitro culture technique
DOI:
https://doi.org/10.1590/S1678-3921.pab1992.v27.3693Keywords:
<i>Cocos nucifera</i>, young plant leaves, adult plant leaves, inflorescence, clonal propagation, tissue culture, callusAbstract
The purpose of this study was to develop a method for vegetative propagation of coconut palm through tissue culture. Young and adult plant leaves and inflorescence were used as explant sources. For the callus induction different concentrations of 6-benzilaminopurine (BAP), 2-isopentyl adenine (2ip) and 2.4-dichlorophenoxyacetic acid (2.4-D) were used. For the shoot induction progressive dosages of BAP were used. Callus was induced on most of the leaf tissues of adult plant by culturing on solid medium (1.1 x 10-4 M 2.4-D), following one week of culture in liquid medium (2.3 x 10-4 M 2.4-D). For inflorescence the best results were obtained with concentrations between 1.1 x 10-4 and 1.7 x 10-4 M in solid medium. Complete callus formation and shoot differentiation was observed on inflorescence explants.
