Cobalt determination in bovine faeces by extraction with 1M HCL

Abstract

A non-digestion HCl method is described for extract Co in bovine faeces, which animals were fed with Co as marker in digest rate. The results were compared with those obtained by conventional dry-ashing and acid digestion techniques. The procedure for the proposed method involves shaking of 1.0 g of the bovine faeces sample with 10 ml of 1MHCl, heating in water bath at 80^oC for 15 minutes, and shaking for 30 minutes. The extract was filtered and Co determined by atomic absorption spectrophotometry. The concentration of Co in the bovine faeces varied from 0 to 170 µ g/g. The proponed method gives Co values that are in close agreement with those obtained with conventional digestion techniques; 98.3% of added Co in the bovine faeces was recovered by the non digestion method, and the variation coefficient was 1.48%. This technique was found to be more rapid, lower cost, not yield toxic gases, eliminate HC1O₄ explosion danger, does not require special laboratory facilities, and of satisfactory precision and accuracy.