Characterization of Nomuraea rileyi isolates through electrophoresis of esterases

Abstract

This work aims at identifying the electrophoretic patterns of alpha- arld beta-esterases isoenzymes from nine isolates of the entomogenous fungus Nomuraea rileyi (Farlow) Samson. The isolates were obtained frorn Anticarsia gemmatalis Huebner (Lep.: Noctuidae) larvae collected in soybean (Glycine max Merrill.) fields in counties located in Rio Grande do Sul, Brazil: Cachoeirinha, Guaiba, Marau, Nova Prata, Palmeira das Missões, Pelotas, Santa Rosa, Tapera, and Taquari. The micelia and conidia of isolates were submitted to electrophoresis using horizontal migration in polyacrylamide gel and continuous electrical current of 10 v/cm and 50 mA/plate. Electrophoretic bands, as well as their relative migrations (RM) and their relative intensities, were recorded. The comparison among isolates was done through phenetic analysis, using mathematical models of Jaceard Association and grouping according to the UPGMA method (unweighted par group method using arithmetic average). It was observed that the differentiation among isolates at esterasic level was quite accentuated, both in location and intensity of bands, permitting the characterization of isolates. Sites of origin of isolates had no influence in the detection of similarities among them.