Multiplication In vitro of apple shoots marubakaido (Malus prunifolia Willd, Borkh) and megumi (Malus domestica Borkh) cultivars

Abstract

Apple shoots, Marubakaido (Malus prunifolia Willd, Borkh) rootstocks, also known as Maruba and Megumi (Malus domestica, Borkh) cultivars, from meristem culture, were multiplied "in vitro" from six different culture media, added benzylaminopurine to BAP (2.0, 3.5 and 5.0) mg/1 and GA₃ (0,0 and 1,0) mg/l. The objective of the experiment was to verify the best culture media for both cultivars. The parameters used were: shoot number, that came from an initial explant, and also the length of those shoots. The best results were obtained with 2,0 mg/1 of BAP, for the rootstock Maruba, and 3,5 mg/1 for the cultivar Megumi. The rate of multiplication and the growing of the shoots varied according to the genotype used.